本研究探討咖啡渣萃取物(WERCR) 對低密度脂蛋白氧化及對peroxynitrite引發SVEC內皮細胞株蛋白硝化作用之影響。咖啡渣萃取物在0.02 - 0.1 mg/ml可以有效減少低密度脂蛋白氧化。另外，咖啡渣萃取物不但可以捕捉一氧化氮自由基，也可以有效捕捉超氧自由基。雖然咖啡渣萃取物無法減少脂多醣(Lipopolysaccharide; LPS)對巨噬細胞株RAW 264.7誘發iNOS及COX-2蛋白表現，但是可以有效減少peroxynitrite對內皮細胞進行蛋白硝化作用，此結果與與彼等捕捉一氧化氮與超氧陰離子有關係。 The effects of water extracts of roasted coffee residue (WERER) on low-density lipoprotein oxidation and peroxynitrite-induced protein nitration in SVEC endothelium cells were investigated. At 0.02~0.1 mg/ml, WERER showed a protective role on low-density lipoprotein oxidation. Furthermore,WERER not only directly scavenged nitric oxide but also scavenged superoxide. WERER could not affect iNOS and cyclooxygenase-2 protein expression in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. On the other hand, WERER displayed obviously inhibition effects on peroxynitrite-induced protein nitration in endothelium cells.
