The aim of this study is to investigate the djulis and its bioactive compounds to prevent blue light (BL)-induced photo-oxidative stress in ARPE-19 cells. After 6 h of BL exposure (peak at 452 nm), ARPE-19 cell viability significantly decreased, but treatment with water extract of djulis (WECF) at 0.1-25 mu g/mL protected the cells from BL-induced phototoxicity. The cytoprotective effect of WECF were associated with a decrease in BL-induced oxidative stress and caspase-3 activity, and an increase in GSH levels and SOD activity. WECF also inhibited the expression of NF-kappa B protein and the MAPK signaling pathway, while upregulated heme oxygenase-1 (HO-1) via the Nrf2-mediated pathway. Moreover, betanin, kaempferol, quercetin and rutin, present in WECF, showed a protective effect against BL-induced photo-oxidative stress at 1-10 mu M. Overall, WECF and its bioactive com-pounds have the potential to protect ARPE-19 cells from BL-induced retina damage, which could have thera-peutic implications for retinal disease.
關聯:
Journal of Functional Foods, v.109, Article 105797
