Establishing and maintaining colonies of Forcipomyia taiwana in the laboratory

doi:10.1111/jvec.12317

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 期刊論文 > Item 310902800/32277

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標題:	Establishing and maintaining colonies of Forcipomyia taiwana in the laboratory
作者:	Luo, Yi-Pey
貢獻者:	Chia Nan Univ Pharm & Sci, Dept Biotechnol
關鍵字:	Forcipomyia taiwana artificial blood-feeding larvae rearing swarming and copulation mating rates
日期:	2018-12
上傳時間:	2019-11-15 15:47:50 (UTC+8)
出版者:	SOC VECTOR ECOLOGY
摘要:	Successful colonies of the biting midge Forcipomyia taiwana (Shiraki) were established and maintained in the laboratory by feeding blood with an artificial blood-feeding apparatus, rearing larvae on a soil substrate employing algae liquid, and setting suitable mating cages. The feeding rates of F. taiwana fed on pig blood (69.9%) and artificial blood (72.7%) were not significantly different from those fed on human blood (67.0%). The mean numbers of adults produced by females fed on the artificial blood and the human blood were 32.0 and 33.0, respectively. The algae liquid, Chlorella vulgaris, was suitable for rearing larvae, with larval hatching rate, pupation rate, and emergence rate of midges fed with artificial blood and human blood meal cohorts observed as 76.0%-88.8%, 98.2%-96.4%, and 98.0-94.3%, respectively. Swarming and copulation occurred 1 h before and 2 h after the lights were turned on (07:00-10:00). The average female mating rates were approximately 50-60%, and males were observed to mate with multiple females.
link:	http://dx.doi.org/10.1111/jvec.12317
關聯:	International Journal of Molecular Medicine, v.43, n.2, pp.328-333
显示于类别:	[生物科技系(所)] 期刊論文

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