Establishing and maintaining colonies of Forcipomyia taiwana in the laboratory

doi:10.1111/jvec.12317

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/32277

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/32277

Title:	Establishing and maintaining colonies of Forcipomyia taiwana in the laboratory
Authors:	Luo, Yi-Pey
Contributors:	Chia Nan Univ Pharm & Sci, Dept Biotechnol
Keywords:	Forcipomyia taiwana artificial blood-feeding larvae rearing swarming and copulation mating rates
Date:	2018-12
Issue Date:	2019-11-15 15:47:50 (UTC+8)
Publisher:	SOC VECTOR ECOLOGY
Abstract:	Successful colonies of the biting midge Forcipomyia taiwana (Shiraki) were established and maintained in the laboratory by feeding blood with an artificial blood-feeding apparatus, rearing larvae on a soil substrate employing algae liquid, and setting suitable mating cages. The feeding rates of F. taiwana fed on pig blood (69.9%) and artificial blood (72.7%) were not significantly different from those fed on human blood (67.0%). The mean numbers of adults produced by females fed on the artificial blood and the human blood were 32.0 and 33.0, respectively. The algae liquid, Chlorella vulgaris, was suitable for rearing larvae, with larval hatching rate, pupation rate, and emergence rate of midges fed with artificial blood and human blood meal cohorts observed as 76.0%-88.8%, 98.2%-96.4%, and 98.0-94.3%, respectively. Swarming and copulation occurred 1 h before and 2 h after the lights were turned on (07:00-10:00). The average female mating rates were approximately 50-60%, and males were observed to mate with multiple females.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1111/jvec.12317
Relation:	International Journal of Molecular Medicine, v.43, n.2, pp.328-333
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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