Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/32252
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    標題: RNA-seq transcriptome analysis of breast cancer cell lines under shikonin treatment
    作者: Lin, Kuo-Hua
    Huang, Ming-Yii
    Cheng, Wei-Chung
    Wang, Shu-Chi
    Fang, Shih-Hua
    Tu, Hung-Pin
    Su, Chia-Cheng
    Hung, Yung-Li
    Liu, Po-Len
    Chen, Chi-Shuo
    Wang, Yu-Ting
    Li, Chia-Yang
    貢獻者: Changhua Christian Hosp, Dept Surg
    Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Ctr Canc, Dept Radiat Oncol
    China Med Univ, Grad Inst Biomed Sci
    Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Hlth Management Ctr
    Natl Taiwan Univ Sport, Inst Athlet
    Kaohsiung Med Univ, Coll Med, Sch Med, Dept Publ Hlth & Environm Med
    Chi Mei Med Ctr, Dept Surg, Div Urol
    Kaohsiung Med Univ, Grad Inst Med, Coll Med
    Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management
    Waseda Univ, Grad Sch Sport Sci
    Kaohsiung Med Univ, Coll Med, Dept Resp Therapy
    Natl Tsing Hua Univ, Dept Biomed Engn & Environm Sci
    Kaohsiung Med Univ, Ctr Infect Dis & Canc Res
    Kaohsiung Med Univ Hosp, Dept Med Res
    關鍵字: In-Vitro
    Lithospermum-Erythrorhizon
    Signaling Pathways
    Expression
    Database
    Proliferation
    Angiogenesis
    Integration
    Metastasis
    Ingredient
    日期: 2018-02-08
    上傳時間: 2019-11-15 15:46:53 (UTC+8)
    出版者: NATURE PUBLISHING GROUP
    摘要: Shikonin is a naphthoquinone isolated from the dried root of Lithospermum erythrorhizon, an herb used in Chinese medicine. Although several studies have indicated that shikonin exhibits antitumor activity in breast cancer, the mechanism of action remains unclear. In the present study, we performed transcriptome analysis using RNA-seq and explored the mechanism of action of shikonin in regulating the growth of different types of breast cancer cells. The IC50 of shikonin on MCF-7, SKBR-3 and MDA-MB-231 cells were 10.3 mu M, 15.0 mu M, 15.0 mu M respectively. Our results also demonstrated that shikonin arrests the progression of cell cycle and induces apoptosis in MDA-MB-231 cells. Using RNA-seq transcriptome analysis, we found 38 common genes that significantly express in different types of breast cancer cells under shikonin treatment. In particular, our results indicated that shikonin induces the expression of dual specificity phosphatase (DUSP)-1 and DUSP2 in both RNA and protein levels. In addition, shikonin also inhibits the phosphorylation of JNK and p38, the downstream signaling molecules of DUSP1 and DUSP2. Therefore, our results suggest that shikonin induces the expression of DUSP1 and DUSP2 which consequently switches off JNK and p38 MAPK pathways and causes cell cycle arrest and apoptosis in breast cancer cells.
    link: http://dx.doi.org/10.1038/s41598-018-21065-x
    關聯: Scientific Reports, v.8, 2672
    顯示於類別:[老人服務事業管理系] 期刊論文

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