Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/32252
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 17760/20057 (89%)
造访人次 : 7975252      在线人数 : 586
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cnu.edu.tw/handle/310902800/32252


    標題: RNA-seq transcriptome analysis of breast cancer cell lines under shikonin treatment
    作者: Lin, Kuo-Hua
    Huang, Ming-Yii
    Cheng, Wei-Chung
    Wang, Shu-Chi
    Fang, Shih-Hua
    Tu, Hung-Pin
    Su, Chia-Cheng
    Hung, Yung-Li
    Liu, Po-Len
    Chen, Chi-Shuo
    Wang, Yu-Ting
    Li, Chia-Yang
    貢獻者: Changhua Christian Hosp, Dept Surg
    Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Ctr Canc, Dept Radiat Oncol
    China Med Univ, Grad Inst Biomed Sci
    Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Hlth Management Ctr
    Natl Taiwan Univ Sport, Inst Athlet
    Kaohsiung Med Univ, Coll Med, Sch Med, Dept Publ Hlth & Environm Med
    Chi Mei Med Ctr, Dept Surg, Div Urol
    Kaohsiung Med Univ, Grad Inst Med, Coll Med
    Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management
    Waseda Univ, Grad Sch Sport Sci
    Kaohsiung Med Univ, Coll Med, Dept Resp Therapy
    Natl Tsing Hua Univ, Dept Biomed Engn & Environm Sci
    Kaohsiung Med Univ, Ctr Infect Dis & Canc Res
    Kaohsiung Med Univ Hosp, Dept Med Res
    關鍵字: In-Vitro
    Lithospermum-Erythrorhizon
    Signaling Pathways
    Expression
    Database
    Proliferation
    Angiogenesis
    Integration
    Metastasis
    Ingredient
    日期: 2018-02-08
    上傳時間: 2019-11-15 15:46:53 (UTC+8)
    出版者: NATURE PUBLISHING GROUP
    摘要: Shikonin is a naphthoquinone isolated from the dried root of Lithospermum erythrorhizon, an herb used in Chinese medicine. Although several studies have indicated that shikonin exhibits antitumor activity in breast cancer, the mechanism of action remains unclear. In the present study, we performed transcriptome analysis using RNA-seq and explored the mechanism of action of shikonin in regulating the growth of different types of breast cancer cells. The IC50 of shikonin on MCF-7, SKBR-3 and MDA-MB-231 cells were 10.3 mu M, 15.0 mu M, 15.0 mu M respectively. Our results also demonstrated that shikonin arrests the progression of cell cycle and induces apoptosis in MDA-MB-231 cells. Using RNA-seq transcriptome analysis, we found 38 common genes that significantly express in different types of breast cancer cells under shikonin treatment. In particular, our results indicated that shikonin induces the expression of dual specificity phosphatase (DUSP)-1 and DUSP2 in both RNA and protein levels. In addition, shikonin also inhibits the phosphorylation of JNK and p38, the downstream signaling molecules of DUSP1 and DUSP2. Therefore, our results suggest that shikonin induces the expression of DUSP1 and DUSP2 which consequently switches off JNK and p38 MAPK pathways and causes cell cycle arrest and apoptosis in breast cancer cells.
    link: http://dx.doi.org/10.1038/s41598-018-21065-x
    關聯: Scientific Reports, v.8, 2672
    显示于类别:[老人服務事業管理系] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    10.1038-s41598-018-21065-x.pdf2675KbAdobe PDF47检视/开启
    index.html0KbHTML168检视/开启


    在CNU IR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈