RNA-seq transcriptome analysis of breast cancer cell lines under shikonin treatment

doi:10.1038/s41598-018-21065-x

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Title:	RNA-seq transcriptome analysis of breast cancer cell lines under shikonin treatment
Authors:	Lin, Kuo-Hua Huang, Ming-Yii Cheng, Wei-Chung Wang, Shu-Chi Fang, Shih-Hua Tu, Hung-Pin Su, Chia-Cheng Hung, Yung-Li Liu, Po-Len Chen, Chi-Shuo Wang, Yu-Ting Li, Chia-Yang
Contributors:	Changhua Christian Hosp, Dept Surg Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Ctr Canc, Dept Radiat Oncol China Med Univ, Grad Inst Biomed Sci Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Hlth Management Ctr Natl Taiwan Univ Sport, Inst Athlet Kaohsiung Med Univ, Coll Med, Sch Med, Dept Publ Hlth & Environm Med Chi Mei Med Ctr, Dept Surg, Div Urol Kaohsiung Med Univ, Grad Inst Med, Coll Med Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management Waseda Univ, Grad Sch Sport Sci Kaohsiung Med Univ, Coll Med, Dept Resp Therapy Natl Tsing Hua Univ, Dept Biomed Engn & Environm Sci Kaohsiung Med Univ, Ctr Infect Dis & Canc Res Kaohsiung Med Univ Hosp, Dept Med Res
Keywords:	In-Vitro Lithospermum-Erythrorhizon Signaling Pathways Expression Database Proliferation Angiogenesis Integration Metastasis Ingredient
Date:	2018-02-08
Issue Date:	2019-11-15 15:46:53 (UTC+8)
Publisher:	NATURE PUBLISHING GROUP
Abstract:	Shikonin is a naphthoquinone isolated from the dried root of Lithospermum erythrorhizon, an herb used in Chinese medicine. Although several studies have indicated that shikonin exhibits antitumor activity in breast cancer, the mechanism of action remains unclear. In the present study, we performed transcriptome analysis using RNA-seq and explored the mechanism of action of shikonin in regulating the growth of different types of breast cancer cells. The IC50 of shikonin on MCF-7, SKBR-3 and MDA-MB-231 cells were 10.3 mu M, 15.0 mu M, 15.0 mu M respectively. Our results also demonstrated that shikonin arrests the progression of cell cycle and induces apoptosis in MDA-MB-231 cells. Using RNA-seq transcriptome analysis, we found 38 common genes that significantly express in different types of breast cancer cells under shikonin treatment. In particular, our results indicated that shikonin induces the expression of dual specificity phosphatase (DUSP)-1 and DUSP2 in both RNA and protein levels. In addition, shikonin also inhibits the phosphorylation of JNK and p38, the downstream signaling molecules of DUSP1 and DUSP2. Therefore, our results suggest that shikonin induces the expression of DUSP1 and DUSP2 which consequently switches off JNK and p38 MAPK pathways and causes cell cycle arrest and apoptosis in breast cancer cells.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1038/s41598-018-21065-x
Relation:	Scientific Reports, v.8, 2672
Appears in Collections:	[Dept. of Senior Service and Health Management] Periodical Articles

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