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| 標題: | RNA-seq transcriptome analysis of breast cancer cell lines under shikonin treatment |
| 作者: | Lin, Kuo-Hua
Huang, Ming-Yii
Cheng, Wei-Chung
Wang, Shu-Chi
Fang, Shih-Hua
Tu, Hung-Pin
Su, Chia-Cheng
Hung, Yung-Li
Liu, Po-Len
Chen, Chi-Shuo
Wang, Yu-Ting
Li, Chia-Yang |
| 貢獻者: | Changhua Christian Hosp, Dept Surg
Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Ctr Canc, Dept Radiat Oncol
China Med Univ, Grad Inst Biomed Sci
Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Hlth Management Ctr
Natl Taiwan Univ Sport, Inst Athlet
Kaohsiung Med Univ, Coll Med, Sch Med, Dept Publ Hlth & Environm Med
Chi Mei Med Ctr, Dept Surg, Div Urol
Kaohsiung Med Univ, Grad Inst Med, Coll Med
Chia Nan Univ Pharm & Sci, Dept Senior Citizen Serv Management
Waseda Univ, Grad Sch Sport Sci
Kaohsiung Med Univ, Coll Med, Dept Resp Therapy
Natl Tsing Hua Univ, Dept Biomed Engn & Environm Sci
Kaohsiung Med Univ, Ctr Infect Dis & Canc Res
Kaohsiung Med Univ Hosp, Dept Med Res |
| 關鍵字: | In-Vitro
Lithospermum-Erythrorhizon
Signaling Pathways
Expression
Database
Proliferation
Angiogenesis
Integration
Metastasis
Ingredient |
| 日期: | 2018-02-08 |
| 上傳時間: | 2019-11-15 15:46:53 (UTC+8) |
| 出版者: | NATURE PUBLISHING GROUP |
| 摘要: | Shikonin is a naphthoquinone isolated from the dried root of Lithospermum erythrorhizon, an herb used in Chinese medicine. Although several studies have indicated that shikonin exhibits antitumor activity in breast cancer, the mechanism of action remains unclear. In the present study, we performed transcriptome analysis using RNA-seq and explored the mechanism of action of shikonin in regulating the growth of different types of breast cancer cells. The IC50 of shikonin on MCF-7, SKBR-3 and MDA-MB-231 cells were 10.3 mu M, 15.0 mu M, 15.0 mu M respectively. Our results also demonstrated that shikonin arrests the progression of cell cycle and induces apoptosis in MDA-MB-231 cells. Using RNA-seq transcriptome analysis, we found 38 common genes that significantly express in different types of breast cancer cells under shikonin treatment. In particular, our results indicated that shikonin induces the expression of dual specificity phosphatase (DUSP)-1 and DUSP2 in both RNA and protein levels. In addition, shikonin also inhibits the phosphorylation of JNK and p38, the downstream signaling molecules of DUSP1 and DUSP2. Therefore, our results suggest that shikonin induces the expression of DUSP1 and DUSP2 which consequently switches off JNK and p38 MAPK pathways and causes cell cycle arrest and apoptosis in breast cancer cells. |
| link: | http://dx.doi.org/10.1038/s41598-018-21065-x |
| 關聯: | Scientific Reports, v.8, 2672 |
| 顯示於類別: | [高齡福祉養生管理系] 期刊論文
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