English  |  正體中文  |  简体中文  |  Items with full text/Total items : 18076/20274 (89%)
Visitors : 4817473      Online Users : 756
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/28506


    標題: Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells
    作者: Chen, Ying-hui
    Hao, Lyh-Jyh
    Hung, Chih-peng
    Chen, Jung-wei
    Leu, Sew-fen
    Huang, Bu-miin
    貢獻者: 溫泉產業研究所
    關鍵字: cisplatin
    cordycepin
    apoptosis
    OC3
    synergistic
    日期: 2014-08
    上傳時間: 2015-05-06 21:18:45 (UTC+8)
    出版者: Springer
    摘要: To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. The influences of cisplatin (2.5 mu g/mL) and/or cordycepin treatment (10 or 100 mu mol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Data demonstrated that co-administration of cisplatin (2.5 mu g/mL) and cordycepin (10 or 100 mu mol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P < 0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P < 0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P < 0.05). Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
    關聯: Chinese Journal of Integrative Medicine, v.20 n.8, pp.624-632
    Appears in Collections:[觀光事業管理系(含溫泉所)] 期刊論文

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML1979View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback