Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/28506
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    Title: Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells
    Authors: Chen, Ying-hui
    Hao, Lyh-Jyh
    Hung, Chih-peng
    Chen, Jung-wei
    Leu, Sew-fen
    Huang, Bu-miin
    Contributors: 溫泉產業研究所
    Keywords: cisplatin
    cordycepin
    apoptosis
    OC3
    synergistic
    Date: 2014-08
    Issue Date: 2015-05-06 21:18:45 (UTC+8)
    Publisher: Springer
    Abstract: To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. The influences of cisplatin (2.5 mu g/mL) and/or cordycepin treatment (10 or 100 mu mol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Data demonstrated that co-administration of cisplatin (2.5 mu g/mL) and cordycepin (10 or 100 mu mol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P < 0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P < 0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P < 0.05). Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
    Relation: Chinese Journal of Integrative Medicine, v.20 n.8, pp.624-632
    Appears in Collections:[Dept. of Tourism Management] Periodical Articles

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