Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells

doi:10.1007/s11655-013-1453-3

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Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/28506

Title:	Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells
Authors:	Chen, Ying-hui Hao, Lyh-Jyh Hung, Chih-peng Chen, Jung-wei Leu, Sew-fen Huang, Bu-miin
Contributors:	溫泉產業研究所
Keywords:	cisplatin cordycepin apoptosis OC3 synergistic
Date:	2014-08
Issue Date:	2015-05-06 21:18:45 (UTC+8)
Publisher:	Springer
Abstract:	To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. The influences of cisplatin (2.5 mu g/mL) and/or cordycepin treatment (10 or 100 mu mol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Data demonstrated that co-administration of cisplatin (2.5 mu g/mL) and cordycepin (10 or 100 mu mol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P < 0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P < 0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P < 0.05). Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
Relation:	Chinese Journal of Integrative Medicine, v.20 n.8, pp.624-632
Appears in Collections:	[Dept. of Tourism Management] Periodical Articles

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