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https://ir.cnu.edu.tw/handle/310902800/28506
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| 標題: | Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells |
| 作者: | Chen, Ying-hui
Hao, Lyh-Jyh
Hung, Chih-peng
Chen, Jung-wei
Leu, Sew-fen
Huang, Bu-miin |
| 貢獻者: | 溫泉產業研究所 |
| 關鍵字: | cisplatin
cordycepin
apoptosis
OC3
synergistic |
| 日期: | 2014-08 |
| 上傳時間: | 2015-05-06 21:18:45 (UTC+8) |
| 出版者: | Springer |
| 摘要: | To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. The influences of cisplatin (2.5 mu g/mL) and/or cordycepin treatment (10 or 100 mu mol/L) to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium (MTT) assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Data demonstrated that co-administration of cisplatin (2.5 mu g/mL) and cordycepin (10 or 100 mu mol/L) resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycepin alone treatment (24 h), respectively (P < 0.05). In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin (30%) was significantly higher than cisplatin (5%) or cordycepin (12%) alone group (P < 0.05), confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/Jun terminal kinase (JNK), the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase (PARP) as compared to cisplatin or cordycepin alone treatment (P < 0.05). Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line. |
| 關聯: | Chinese Journal of Integrative Medicine, v.20 n.8, pp.624-632 |
| 显示于类别: | [觀光事業管理系(含溫泉所)] 期刊論文
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