以魚類肝臟初代細胞株探討環境中仿雌激素物質的可行性研究─總計劃

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Title:	以魚類肝臟初代細胞株探討環境中仿雌激素物質的可行性研究─總計劃
Other Titles:	子計劃1 : 都市處理廠放流水中仿雌激素活性之探討子計畫2 : 人工濕地放流水仿雌激素活性之探討子計畫3 : 底泥孔隙水中仿雌激素活性之探討
Authors:	陳健民
Contributors:	環境資源管理系
Keywords:	仿雌激素間隙水底泥 xenoestrogen interstitial water sediment
Date:	2004
Issue Date:	2008-06-20 13:53:25 (UTC+8)
Publisher:	台南縣：嘉南藥理科技大學環境資源管理系
Abstract:	由於界面活性劑、殺蟲劑及塑化劑等物質之運用，使全球各地水體皆發現有環境荷爾蒙物質，此類物質會有類似一般生物體內荷爾蒙物質之作用，改變正常功能，亦稱為內分泌干擾物(endocrine disrupting chemicals， EDCs)，其對生物及人類之影響已引起注意。在環境荷爾蒙物質中最常被討論的物質為仿雌激素，其亦常出現於污染水域環境中，逐漸蓄積於底泥系統間隙水中而成為一污染源，並藉由分佈平衡現象而釋出回到水體。因此，以生物指標方法檢視環境中的此類物質即成為目前環境污染凡至工作之重要課題之一。本整合計畫採取不同的環境樣本，即經人工濕地系統處理後之進流與放流水、都市處理廠放流水、污染河川底泥的孔隙水等三種環境水樣，以青將魚(Japanese Medaka, Oryzias latips) 肝臟培養細胞中卵黃蛋白先質(vitellogenin, VTG）的表現檢視其雌激素的活性。實驗結果顯示除底泥間隙水水樣無法測試外，暴露於其他測試水皆有VTG 的表現，並與暴露時間之長短有相關。 There are many EDCs found in the water around the world because of the usage of surfactants 、pesticides and plasticizer. These chemicals can disrupt normal hormonal functions in human and other organisms. These effects have already caused a lot of attention. Xenoestrogen is one of EDCs can be found in polluted water as well as in the sediment-system. Once xenoestrogen are transported into the sediments, the system acts as a reservoir and xenoestrogen can be released back to the water because the partitioning effect. Therefore, developing biomarkers to detect such EDCs in different environmental matrix became a major issue. The purpose of this project was to examine the activity of xenoestrogen in the different environmental samples, namely effluents from a constructed wetland, a municipal sewage treatment plant, and porewater extracted from sediment of a polluted river. We used expression of vitillogen (VTG) from cultured primary hepatocytes from Japanese medaka, Oryzias latips, as the biomarker. Results show that, except porewater could not have significant outcome due to contamination of the culture cells, other water samples tested led to expression of VTG in exposed hepatocytes, and the productions was time-dependent.
Relation:	計畫編號：CNEM9301
Appears in Collections:	[Dept. of Environmental Resources Management] Chna Project

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