利用免疫二維電泳研發家禽霍亂重組蛋白質疫苗(I)

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標題:	利用免疫二維電泳研發家禽霍亂重組蛋白質疫苗(I) Development of Fowl Cholera Recombinant Vaccine by Two-Dimensional Immunoelectrophoresis
作者:	邱淑媛吳定峰
貢獻者:	保健營養系行政院農業委員會
關鍵字:	免疫二維電泳巴斯德桿菌家禽霍亂免疫原 Two Dimensional Immunoelectrophoresis Pasteurella multocida Fowl cholera Immunogen
日期:	2007
上傳時間:	2008-11-21 10:35:14 (UTC+8)
摘要:	本研究的目的在解決家禽霍亂疫苗保護力不佳的問題，藉由研發一新型的重組次單位疫苗，改善家禽霍亂疫苗的效力。利用二維電泳分離抗原，配合以不同保護力疫苗所免疫的抗血清進行免疫染色，鑑定菌體中的重要蛋白質抗原，再進而利用這些免疫原建構重組蛋白，研發次單位疫苗。 The aim of this study is to develop a novel recombinant subunit vaccine to improve the efficacy of vaccine against fowl cholera. The study is proceeded by identify high immunogenic proteinaceous fowl cholera-specific antigens on Pasteurella multocida by performing immunoblotting after two-dimensional polyacrylamide gel electrophoresis (2D PAGE). The important immunogenetic proteins are recognized by antibodies of mice immunized with high-protective vaccine but not by sera from mice immunized with low-efficacy vaccine. Those proteinaceous immunogen candidates are identified by subjected to trypsin digestion followed by peptide mass fingerprinting and GeneBank search. In the second year, these genes will then be cloned and transformed to Escherichia coli for protein expression. In the third year, vaccine are prepared with these recombinant proteins and their efficacy are examined by challenge experiments in mice.
關聯:	計畫編號：96農科-1.1.5-檢-B1(6)
顯示於類別:	[保健營養系(所) ] 其他研究計畫

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