本研究擬以兩年時間完成三項主要工作： (1) 將93年度科技計畫中完成的多殺性巴斯德桿菌高密度液態培養平台放大製產業化層級， (2) 降低培養基成本， (3) 建立醱酵槽連續培養技術或高密度培養的技術。期能藉由提高菌液濃度、降低物料成本與引進醱酵程序自動控制的技術節約人力成本三方面，協助過內業者產製優質疫苗，提高疫苗產業的國際競爭力。 There are three aims of this study to be achieved in two years. (1) To scale up the high-density culture technique from the laboratory scale (which was developed for Pasteurella multocida with the financial supports from the Council of Agriculture, ROC in 2004) to a commercial scale, for better cell recovery than the commercially culture system being in use in vaccine production. (2) Lower the cost of culture medium by substituting certain of the components with fermentation-grade substrates. (3) To setup a continuous culture technique or a high-density culture technique in the fermenter with automatically feedback control by signals from the cultivation, to save the cost of manpower.
