本研究目的在於建立植酸、甘醇酸、乳酸、蘋果酸與杏仁酸之混酸溶液之分析方法。研究中針對管柱的種類、移動相之種類與組成、移動相之流速等因素。對分析物層析結果之影響進行探討，以找到最適化的分離條件。研究中先對分析物個別分析，找到適當的條件再進行混酸之分析。研究結果發現以Discovery® C18 (250×4.6mm，5μm)為固定相，磷酸緩衝液（pH2）和甲醇之混合溶液（95：5）為移動相，流速為0.5 ml/min時，可得到最佳的分離結果。此方法應可應用之實際樣品之分析。 The aim of this research is to establish a analytical method for the determination of phytic acid , glycolic acid , lactic acid , malic acid and mandelic acid in cosmetics.The following factors were considered in this research；column type , pH buffer and organic modifier in the mobile phase and the flow rate of mobile phase.
The use of Discovery® C18 column as the stationary phase and a mixture of phosphate buffer(pH2 ) and methanol (95: 5) should be a better choice for the determination of phytic acid and fruit acid in cosmetics.
The results could be applied to the determination of phytic acid and fruit acid in real sample.
