A graphene oxide/polyaniline nanocomposite biosensor: synthesis, characterization, and electrochemical detection of bilirubin

doi:10.1039/d3ra06815c

CNU IR > Pharmacy and Science > Dept. of Pharmacy > Periodical Articles > Item 310902800/34928

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34928

Title:	A graphene oxide/polyaniline nanocomposite biosensor: synthesis, characterization, and electrochemical detection of bilirubin
Authors:	Ahmed, Noor sabah Hsu, Chou-Yi Mahmoud, Zaid H. Sayadi, Hamidreza Kianfar, Ehsan
Contributors:	Univ Diyala, Coll Sci Chia Nan Univ Pharm & Sci, Dept Pharm Univ Diyala, Coll Sci, Chem Dept Fac Shahrood Branch, Dept Chem Engn, Shahrood Branch Islamic Azad Univ, Dept Chem Engn, Arak Branch Islamic Azad Univ, Gachsaran Branch, Young Researchers & Elite Club
Keywords:	Sensitive Detection Oxide Nanosheets Membranes Serum Nanoparticles Separation Sensor
Date:	2023
Issue Date:	2024-12-25 11:05:47 (UTC+8)
Publisher:	ROYAL SOC CHEMISTRY
Abstract:	The level of free bilirubin is a considerable index for the characterization of jaundice-related diseases. Herein, a biosensor was fabricated via the immobilization of bilirubin oxidase (BOx) on graphene oxide (GO) and polyaniline (PANI) that were electrochemically co-precipitated on indium tin oxide (ITO) conductive glass. The structural enzyme electrode was characterized by FTIR, XRD, and Raman spectroscopy, while the spectral and thermal properties were investigated by UV-vis and thermogravimetric analysis (TGA). Owing to the activity of the fabricated BOx/GO@PANI/ITO biosensor, it could detect free bilirubin with good selectivity and sensitivity in a low response time. The electrochemical response was studied using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). At polarization potential 0.2 V vs. Ag/AgCl, the fabricated sensor illustrated a response in only 2 s at 30 degrees C and pH 7.5. The LOD and LOQ for the BOx/GO@PANI/ITO biosensor were calculated and found to be 0.15 nM and 2.8 nM, respectively. The electrochemical signal showed a linear response in the concentration range 0.01-250 mu M. At 5 degrees C, the biosensor demonstrated a half-time of 120 days, through which it could be utilized 100 times at this temperature conditions. By using a common colorimetric method, the data on bilirubin levels in serum showed a determination coefficient (R2) of 0.97. The level of free bilirubin is a considerable index for the characterization of jaundice-related diseases.
Relation:	Rsc Advances, v.13, n.51, pp.36280-36292
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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