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https://ir.cnu.edu.tw/handle/310902800/34928
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| 標題: | A graphene oxide/polyaniline nanocomposite biosensor: synthesis, characterization, and electrochemical detection of bilirubin |
| 作者: | Ahmed, Noor sabah
Hsu, Chou-Yi
Mahmoud, Zaid H.
Sayadi, Hamidreza
Kianfar, Ehsan |
| 貢獻者: | Univ Diyala, Coll Sci
Chia Nan Univ Pharm & Sci, Dept Pharm
Univ Diyala, Coll Sci, Chem Dept
Fac Shahrood Branch, Dept Chem Engn, Shahrood Branch
Islamic Azad Univ, Dept Chem Engn, Arak Branch
Islamic Azad Univ, Gachsaran Branch, Young Researchers & Elite Club |
| 關鍵字: | Sensitive Detection
Oxide Nanosheets
Membranes
Serum
Nanoparticles
Separation
Sensor |
| 日期: | 2023 |
| 上傳時間: | 2024-12-25 11:05:47 (UTC+8) |
| 出版者: | ROYAL SOC CHEMISTRY |
| 摘要: | The level of free bilirubin is a considerable index for the characterization of jaundice-related diseases. Herein, a biosensor was fabricated via the immobilization of bilirubin oxidase (BOx) on graphene oxide (GO) and polyaniline (PANI) that were electrochemically co-precipitated on indium tin oxide (ITO) conductive glass. The structural enzyme electrode was characterized by FTIR, XRD, and Raman spectroscopy, while the spectral and thermal properties were investigated by UV-vis and thermogravimetric analysis (TGA). Owing to the activity of the fabricated BOx/GO@PANI/ITO biosensor, it could detect free bilirubin with good selectivity and sensitivity in a low response time. The electrochemical response was studied using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). At polarization potential 0.2 V vs. Ag/AgCl, the fabricated sensor illustrated a response in only 2 s at 30 degrees C and pH 7.5. The LOD and LOQ for the BOx/GO@PANI/ITO biosensor were calculated and found to be 0.15 nM and 2.8 nM, respectively. The electrochemical signal showed a linear response in the concentration range 0.01-250 mu M. At 5 degrees C, the biosensor demonstrated a half-time of 120 days, through which it could be utilized 100 times at this temperature conditions. By using a common colorimetric method, the data on bilirubin levels in serum showed a determination coefficient (R2) of 0.97. The level of free bilirubin is a considerable index for the characterization of jaundice-related diseases. |
| 關聯: | Rsc Advances, v.13, n.51, pp.36280-36292 |
| 顯示於類別: | [藥學系(所)] 期刊論文
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