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| 標題: | Molecular Detection of Rickettsia hoogstraalii in Hyalomma anatolicum and Haemaphysalis sulcata: Updated Knowledge on the Epidemiology of Tick-Borne Rickettsia hoogstraalii |
| 作者: | Aneela, Aneela
Almutairi, Mashal M.
Alouffi, Abdulaziz
Ahmed, Haroon
Tanaka, Tetsuya
Vaz, Itabajara da Silva
Chang, Shun-Chung
Chen, Chien-Chin
Ali, Abid |
| 貢獻者: | Abdul Wali Khan Univ Mardan, Dept Zool
King Saud Univ, Coll Pharm, Dept Pharmacol & Toxicol
King Abdulaziz City Sci & Technol
COMSATS Univ Islamabad CUI, Dept Biosci, Pk Rd
Kagoshima Univ, Joint Fac Vet Med, Lab Infect Dis
Univ Fed Rio Grande do Sul, Ctr Biotecnol
Univ Fed Rio Grande do Sul, Fac Vet
Ditmanson Med Fdn Chia Yi Christian Hosp, Dept Emergency Med
Ditmanson Med Fdn Chia Yi Christian Hosp, Dept Pathol
Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
Natl Chung Hsing Univ, Rong Hsing Res Ctr Translat Med, PhD Program Translat Med
Natl Cheng Kung Univ, Coll Biosci & Biotechnol, Dept Biotechnol & Bioind Sci |
| 關鍵字: | ticks
Ixodidae
Rickettsia hoogstraalii
Pakistan |
| 日期: | 2023 |
| 上傳時間: | 2024-12-25 11:03:44 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | Ticks are hematophagous ectoparasites that transmit pathogens to animals and humans. Updated knowledge regarding the global epidemiology of tick-borne Rickettsia hoogstraalii is dispersed, and its molecular detection and genetic characterization are missing in Pakistan. The current study objectives were to molecularly detect and genetically characterize Rickettsia species, especially R. hoogstraalii, in hard ticks infesting livestock in Pakistan, and to provide updated knowledge regarding their global epidemiology. Ticks were collected from livestock, including goats, sheep, and cattle, in six districts of Khyber Pakhtunkhwa (KP) Pakistan. Overall, 183 hosts were examined, of which 134 (73.2%), including goats (number = 39/54, 72.2%), sheep (23/40, 57.5%), and cattle (71/89, 80%) were infested by 823 ticks. The most prevalent tick species was Rhipicephalus microplus (number = 283, 34.3%), followed by Hyalomma anatolicum (223, 27.0%), Rhipicephalus turanicus (122, 14.8%), Haemaphysalis sulcata (104, 12.6%), Haemaphysalis montgomeryi (66, 8.0%), and Haemaphysalis bispinosa (25, 3.03%). A subset of 210 ticks was selected and screened for Rickettsia spp. using PCR-based amplification and subsequent sequencing of rickettsial gltA and ompB fragments. The overall occurrence rate of R. hoogstraalii was 4.3% (number = 9/210). The DNA of Rickettsia was detected in Hy. anatolicum (3/35, 8.5%) and Ha. sulcata (6/49, 12.2%). However, no rickettsial DNA was detected in Rh. microplus (35), Rh. turanicus (35), Ha. montgomeryi (42), and Ha. bispinosa (14). The gltA and ompB fragments showed 99-100% identity with R. hoogstraalii and clustered phylogenetically with the corresponding species from Pakistan, Italy, Georgia, and China. R. hoogstraalii was genetically characterized for the first time in Pakistan and Hy. anatolicum globally. Further studies should be encouraged to determine the role of ticks in the maintenance and transmission of R. hoogstraalii in different hosts. |
| 關聯: | Veterinary Sciences, v.10, n.10, Article 605 |
| 顯示於類別: | [行政單位] 456
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