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請使用永久網址來引用或連結此文件:
https://ir.cnu.edu.tw/handle/310902800/34690
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標題: | Production of Bivalent Subunit Vaccine for Porcine via 2A-like Sequence in Baculovirus Expression Vector System |
作者: | Chen, Ming-Hsiang Varikkodan, Muhammed Muhsin Lin, Ting-Hui Chiang, Chien-Min Sari, Indah Permata Perng, Ming-Der Wu, Tzong-Yuan |
貢獻者: | National Tsing Hua University Chung Yuan Christian University Chung Yuan Christian University Chia Nan University of Pharmacy & Science |
關鍵字: | classical swine-fever fungal immunomodulatory protein multisystemic wasting syndrome flammulina-velutipes circovirus type-2 molecular epidemiology virus pigs disease insect |
日期: | 2022 |
上傳時間: | 2023-12-11 14:05:19 (UTC+8) |
出版者: | MDPI |
摘要: | Classical swine fever virus (CSFV) and porcine circovirus type 2 (PCV2) have caused severe diseases in swine populations worldwide. Here, a polycistronic baculovirus vector was developed to express a bivalent vaccine, consisting of the CSFV-E2 and PCV2-Cap protein, and an immunomodulator protein derived from the Flammulina velutipes, FVE-FIP, as well as the selection marker, green fluorescent protein. The simultaneous expression of the CSFV-E2 and PCV2-Cap protein was mediated by the 2A-like sequence derived from the Perina nuda virus (PnV), while the expression of the FVE-FIP was driven by the internal ribosome entry site (IRES) element derived from the Rhophalosipum padi virus (RhPV). The Western blot analysis result suggested that the CSFV-E2, PCV2-Cap, and FVE-FIP protein were successfully co-expressed by the infected Spodoptera frugiperda IPBL-Sf21 (Sf21) cell line. The extracted cell lysate containing all three recombinant proteins was administered to Balb/C mice with or without the supplementation of Freund's adjuvant. The ELISA analysis of the serum collected from all the immunized groups showed detectable antibodies against CSFV-E2 and PCV2-Cap. Furthermore, the immunized group without the adjuvant supplementation demonstrated a similar level of antibodies to the group with adjuvant supplementation, suggesting the efficiency of the FVE-FIP in enhancing the immune response. These results demonstrated the polycistronic baculovirus vector could be employed to develop bivalent vaccines for pigs. |
關聯: | PROCESSES, v.10, n.CB2, pp.CC2, pp.-, |
顯示於類別: | [行政單位] 123
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