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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34690


    標題: Production of Bivalent Subunit Vaccine for Porcine via 2A-like Sequence in Baculovirus Expression Vector System
    作者: Chen, Ming-Hsiang
    Varikkodan, Muhammed Muhsin
    Lin, Ting-Hui
    Chiang, Chien-Min
    Sari, Indah Permata
    Perng, Ming-Der
    Wu, Tzong-Yuan
    貢獻者: National Tsing Hua University
    Chung Yuan Christian University
    Chung Yuan Christian University
    Chia Nan University of Pharmacy & Science
    關鍵字: classical swine-fever
    fungal immunomodulatory protein
    multisystemic wasting syndrome
    flammulina-velutipes
    circovirus type-2
    molecular epidemiology
    virus
    pigs
    disease
    insect
    日期: 2022
    上傳時間: 2023-12-11 14:05:19 (UTC+8)
    出版者: MDPI
    摘要: Classical swine fever virus (CSFV) and porcine circovirus type 2 (PCV2) have caused severe diseases in swine populations worldwide. Here, a polycistronic baculovirus vector was developed to express a bivalent vaccine, consisting of the CSFV-E2 and PCV2-Cap protein, and an immunomodulator protein derived from the Flammulina velutipes, FVE-FIP, as well as the selection marker, green fluorescent protein. The simultaneous expression of the CSFV-E2 and PCV2-Cap protein was mediated by the 2A-like sequence derived from the Perina nuda virus (PnV), while the expression of the FVE-FIP was driven by the internal ribosome entry site (IRES) element derived from the Rhophalosipum padi virus (RhPV). The Western blot analysis result suggested that the CSFV-E2, PCV2-Cap, and FVE-FIP protein were successfully co-expressed by the infected Spodoptera frugiperda IPBL-Sf21 (Sf21) cell line. The extracted cell lysate containing all three recombinant proteins was administered to Balb/C mice with or without the supplementation of Freund's adjuvant. The ELISA analysis of the serum collected from all the immunized groups showed detectable antibodies against CSFV-E2 and PCV2-Cap. Furthermore, the immunized group without the adjuvant supplementation demonstrated a similar level of antibodies to the group with adjuvant supplementation, suggesting the efficiency of the FVE-FIP in enhancing the immune response. These results demonstrated the polycistronic baculovirus vector could be employed to develop bivalent vaccines for pigs.
    關聯: PROCESSES, v.10, n.CB2, pp.CC2, pp.-,
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