Production of Bivalent Subunit Vaccine for Porcine via 2A-like Sequence in Baculovirus Expression Vector System

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Title:	Production of Bivalent Subunit Vaccine for Porcine via 2A-like Sequence in Baculovirus Expression Vector System
Authors:	Chen, Ming-Hsiang Varikkodan, Muhammed Muhsin Lin, Ting-Hui Chiang, Chien-Min Sari, Indah Permata Perng, Ming-Der Wu, Tzong-Yuan
Contributors:	National Tsing Hua University Chung Yuan Christian University Chung Yuan Christian University Department of Pharmacy, Chia Nan University of Pharmacy & Science
Keywords:	classical swine-fever fungal immunomodulatory protein multisystemic wasting syndrome flammulina-velutipes circovirus type-2 molecular epidemiology virus pigs disease insect
Date:	2022
Issue Date:	2023-12-11 14:05:19 (UTC+8)
Publisher:	MDPI
Abstract:	Classical swine fever virus (CSFV) and porcine circovirus type 2 (PCV2) have caused severe diseases in swine populations worldwide. Here, a polycistronic baculovirus vector was developed to express a bivalent vaccine, consisting of the CSFV-E2 and PCV2-Cap protein, and an immunomodulator protein derived from the Flammulina velutipes, FVE-FIP, as well as the selection marker, green fluorescent protein. The simultaneous expression of the CSFV-E2 and PCV2-Cap protein was mediated by the 2A-like sequence derived from the Perina nuda virus (PnV), while the expression of the FVE-FIP was driven by the internal ribosome entry site (IRES) element derived from the Rhophalosipum padi virus (RhPV). The Western blot analysis result suggested that the CSFV-E2, PCV2-Cap, and FVE-FIP protein were successfully co-expressed by the infected Spodoptera frugiperda IPBL-Sf21 (Sf21) cell line. The extracted cell lysate containing all three recombinant proteins was administered to Balb/C mice with or without the supplementation of Freund's adjuvant. The ELISA analysis of the serum collected from all the immunized groups showed detectable antibodies against CSFV-E2 and PCV2-Cap. Furthermore, the immunized group without the adjuvant supplementation demonstrated a similar level of antibodies to the group with adjuvant supplementation, suggesting the efficiency of the FVE-FIP in enhancing the immune response. These results demonstrated the polycistronic baculovirus vector could be employed to develop bivalent vaccines for pigs.
Relation:	PROCESSES, v.10, n.5, 895
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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