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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34657

    標題: Enzymatic Synthesis of Novel and Highly Soluble Puerarin Glucoside by Deinococcus geothermalis Amylosucrase
    作者: Ding, Hsiou-Yu
    Wang, Tzi-Yuan
    Wu, Jiumn-Yih
    Tsai, Yu-Li
    Chang, Te-Sheng
    貢獻者: Department of Cosmetic Science, Chia Nan University of Pharmacy & Science
    Academia Sinica - Taiwan
    National University Tainan
    關鍵字: glycosides
    日期: 2022
    上傳時間: 2023-12-11 14:03:23 (UTC+8)
    出版者: MDPI
    摘要: Puerarin (daidzein-8-C-glucoside) is an isoflavone isolated from several leguminous plants of the genus Pueraria. Puerarin possesses several pharmacological properties; however, the poor solubility of puerarin limits its applications. To resolve this poor solubility, Deinococcus geothermalis amylosucrase (DgAS) was used to modify puerarin into more soluble derivatives. The results showed that DgAS could biotransform puerarin into a novel compound: puerarin-4 '-O-alpha-glucoside. The biotransformation reaction was manipulated at different temperatures, pH values, sucrose concentrations, reaction times, and enzyme concentrations. The results showed that the optimal reaction condition was biotransformed by 200 mu g/mL DgAS with 20% (w/v) sucrose at pH 6 and incubated at 40 degrees C for 48 h, and the optimal production yield was 35.1%. Puerarin-4 '-O-alpha-glucoside showed 129-fold higher solubility than that of puerarin and, thus, could be further applied for pharmacological use in the future.
    關聯: MOLECULES, v.27, n.13, Article 4074
    Appears in Collections:[化妝品應用與管理系(所)] 期刊論文

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