Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/34657
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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34657


    Title: Enzymatic Synthesis of Novel and Highly Soluble Puerarin Glucoside by Deinococcus geothermalis Amylosucrase
    Authors: Ding, Hsiou-Yu
    Wang, Tzi-Yuan
    Wu, Jiumn-Yih
    Tsai, Yu-Li
    Chang, Te-Sheng
    Contributors: Department of Cosmetic Science, Chia Nan University of Pharmacy & Science
    Academia Sinica - Taiwan
    National University Tainan
    Keywords: glycosides
    glycosylation
    identification
    efficient
    Date: 2022
    Issue Date: 2023-12-11 14:03:23 (UTC+8)
    Publisher: MDPI
    Abstract: Puerarin (daidzein-8-C-glucoside) is an isoflavone isolated from several leguminous plants of the genus Pueraria. Puerarin possesses several pharmacological properties; however, the poor solubility of puerarin limits its applications. To resolve this poor solubility, Deinococcus geothermalis amylosucrase (DgAS) was used to modify puerarin into more soluble derivatives. The results showed that DgAS could biotransform puerarin into a novel compound: puerarin-4 '-O-alpha-glucoside. The biotransformation reaction was manipulated at different temperatures, pH values, sucrose concentrations, reaction times, and enzyme concentrations. The results showed that the optimal reaction condition was biotransformed by 200 mu g/mL DgAS with 20% (w/v) sucrose at pH 6 and incubated at 40 degrees C for 48 h, and the optimal production yield was 35.1%. Puerarin-4 '-O-alpha-glucoside showed 129-fold higher solubility than that of puerarin and, thus, could be further applied for pharmacological use in the future.
    Relation: MOLECULES, v.27, n.13, Article 4074
    Appears in Collections:[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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