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| 標題: | Enhancement of cytotoxicity and induction of apoptosis by cationic nano-liposome formulation of n-butylidenephthalide in breast cancer cells |
| 作者: | Huang, Xiao-Fan
Chang, Kai-Fu
Lin, Yu-Ling
Liao, Kuang-Wen
Hsiao, Chih-Yen
Sheu, Gwo-Tarng
Tsai, Nu-Man |
| 貢獻者: | Chung Shan Med Univ, Inst Med
Chung Shan Med Univ, Dept Med Lab & Biotechnol
Acad Sinica, Agr Biotechnol Res Ctr
Natl Chiao Tung Univ, Dept Biol Sci & Technol
Natl Chiao Tung Univ, Inst Mol Med & Bioengn
Ditmanson Med Fdn Chia Yi Christian Hosp, Div Nephrol, Dept Internal Med
Chia Nan Univ Pharm & Sci, Dept Hosp & Hlth Care Adm
Chung Shan Med Univ Hosp, Clin Lab |
| 關鍵字: | Polycationic liposome containing PEI and polyethylene glycol complex (LPPC)
n-Butylidenephthalide (BP)
Cell apoptosis
Cell cycle
Synergistic effect |
| 日期: | 2021 |
| 上傳時間: | 2023-11-11 12:00:21 (UTC+8) |
| 出版者: | IVYSPRING INT PUBL |
| 摘要: | Breast cancer is the second most common malignancy in women. Current clinical therapy for breast cancer has many disadvantages, including metastasis, recurrence, and poor quality of life. Furthermore, it is necessary to find a new therapeutic drug for breast cancer patients to meet clinical demand. n-Butylidenephthalide (BP) is a natural and hydrophobic compound that can inhibit several tumors. However, BP is unstable in aqueous or protein-rich environments, which reduces the activity of BP. Therefore, we used an LPPC (Lipo-PEG-PEI complex) that can encapsulate both hydrophobic and hydrophilic compounds to improve the limitation of BP. The purpose of this study is to investigate the anti-tumor mechanisms of BP and BP/LPPC and further test the efficacy of BP encapsulated by LPPC on SK-BR-3 cells. BP inhibited breast cancer cell growth, and LPPC encapsulation (BP/LPPC complex) enhanced the cytotoxicity on breast cancer by stabilizing the BP activity and offering endocytic pathways. Additionally, BP and LPPC-encapsulated BP induced cell cycle arrest at the G0/G1 phase and might trigger both extrinsic as well as intrinsic cell apoptosis pathway, resulting in cell death. Moreover, the BP/LPPC complex had a synergistic effect with doxorubicin of enhancing the inhibitory effect on breast cancer cells. Consequently, LPPC-encapsulated BP could improve the anti-cancer effects on breast cancer in vitro. In conclusion, BP exhibited an anti-cancer effect on breast cancer cells, and LPPC encapsulation efficiently improved the cytotoxicity of BP via an acceleration of entrapment efficiency to induce cell cycle block and apoptosis. Furthermore, BP/LPPC exhibited a synergistic effect in combination with doxorubicin. |
| 關聯: | INT J MED SCI, v.18, n.13, pp.2930-2942 |
| 顯示於類別: | [醫務管理系(所)] 期刊論文
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