Enhancement of cytotoxicity and induction of apoptosis by cationic nano-liposome formulation of n-butylidenephthalide in breast cancer cells

doi:10.7150/ijms.51439

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Title:	Enhancement of cytotoxicity and induction of apoptosis by cationic nano-liposome formulation of n-butylidenephthalide in breast cancer cells
Authors:	Huang, Xiao-Fan Chang, Kai-Fu Lin, Yu-Ling Liao, Kuang-Wen Hsiao, Chih-Yen Sheu, Gwo-Tarng Tsai, Nu-Man
Contributors:	Chung Shan Med Univ, Inst Med Chung Shan Med Univ, Dept Med Lab & Biotechnol Acad Sinica, Agr Biotechnol Res Ctr Natl Chiao Tung Univ, Dept Biol Sci & Technol Natl Chiao Tung Univ, Inst Mol Med & Bioengn Ditmanson Med Fdn Chia Yi Christian Hosp, Div Nephrol, Dept Internal Med Chia Nan Univ Pharm & Sci, Dept Hosp & Hlth Care Adm Chung Shan Med Univ Hosp, Clin Lab
Keywords:	Polycationic liposome containing PEI and polyethylene glycol complex (LPPC) n-Butylidenephthalide (BP) Cell apoptosis Cell cycle Synergistic effect
Date:	2021
Issue Date:	2023-11-11 12:00:21 (UTC+8)
Publisher:	IVYSPRING INT PUBL
Abstract:	Breast cancer is the second most common malignancy in women. Current clinical therapy for breast cancer has many disadvantages, including metastasis, recurrence, and poor quality of life. Furthermore, it is necessary to find a new therapeutic drug for breast cancer patients to meet clinical demand. n-Butylidenephthalide (BP) is a natural and hydrophobic compound that can inhibit several tumors. However, BP is unstable in aqueous or protein-rich environments, which reduces the activity of BP. Therefore, we used an LPPC (Lipo-PEG-PEI complex) that can encapsulate both hydrophobic and hydrophilic compounds to improve the limitation of BP. The purpose of this study is to investigate the anti-tumor mechanisms of BP and BP/LPPC and further test the efficacy of BP encapsulated by LPPC on SK-BR-3 cells. BP inhibited breast cancer cell growth, and LPPC encapsulation (BP/LPPC complex) enhanced the cytotoxicity on breast cancer by stabilizing the BP activity and offering endocytic pathways. Additionally, BP and LPPC-encapsulated BP induced cell cycle arrest at the G0/G1 phase and might trigger both extrinsic as well as intrinsic cell apoptosis pathway, resulting in cell death. Moreover, the BP/LPPC complex had a synergistic effect with doxorubicin of enhancing the inhibitory effect on breast cancer cells. Consequently, LPPC-encapsulated BP could improve the anti-cancer effects on breast cancer in vitro. In conclusion, BP exhibited an anti-cancer effect on breast cancer cells, and LPPC encapsulation efficiently improved the cytotoxicity of BP via an acceleration of entrapment efficiency to induce cell cycle block and apoptosis. Furthermore, BP/LPPC exhibited a synergistic effect in combination with doxorubicin.
Relation:	INT J MED SCI, v.18, n.13, pp.2930-2942
Appears in Collections:	[Dept. of Hospital and Health (including master's program)] Periodical Articles

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