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| 標題: | Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering |
| 作者: | Lin, Chih-Hsun
Hsia, Kai
Su, Chih-Kuan
Chen, Chien-Chin
Yeh, Chang-Ching
Ma, Hsu
Lu, Jen-Her |
| 貢獻者: | Taipei Vet Gen Hosp, Dept Surg, Div Plast & Reconstruct Surg
Natl Yang Ming Chiao Tung Univ, Sch Med, Dept Surg
Chia Yi Christian Hosp, Ditmanson Med Fdn, Dept Pathol
Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
Taipei Vet Gen Hosp, Dept Obstet & Gynecol
Natl Yang Ming Chiao Tung Univ, Dept Obstet & Gynecol
Natl Taipei Univ Nursing & Hlth Sci, Dept Nurse Midwifery & Women Hlth
Natl Yang Ming Chiao Tung Univ, Inst Clin Med
Natl Def Med Ctr, Sch Med, Dept Surg Med & Pediat
Taipei Med Univ Hosp, Sect Pediat Cardiol, Dept Pediat |
| 關鍵字: | sonication decellularized human umbilical artery
ultrasonic decellularization
extracellular matrix
biocompatibility
recellularization |
| 日期: | 2021 |
| 上傳時間: | 2023-11-11 11:50:39 (UTC+8) |
| 出版者: | MDPI |
| 摘要: | Decellularized vascular grafts are useful for the construction of biological small-diameter tissue-engineered vascular grafts (<= 6 mm). Traditional chemical decellularization requires a long treatment time, which may damage the structure and alter the mechanical properties. Decellularization using sonication is expected to solve this problem. The aim of this study was to develop an effective decellularization method using ultrasound followed by washing. Different power values of sonication at 40 kHz were tested for 2, 4, and 8 h followed by a washing procedure. The efficacy of sonication of decellularized human umbilical artery (sDHUA) was evaluated via DNA content, histological staining, mechanical properties, and biocompatibility. The sDHUAs were further implanted into rats for up to 90 days and magnetic resonance angiography (MRA) was performed for the implanted grafts. The results demonstrated that treatment of human umbilical artery (HUA) by sonication at ultrasonic power of 204 W for 4 h followed by washing for 24 h in 2% SDS buffer could eliminate more than 90% of cells and retain similar mechanical properties of the HUA. Recellularization was assessed by scanning electron microscopy (SEM), which indicated that sDHUA provided niches for human umbilical vein endothelial cells (HUVECs) to reside, indicating in vitro cytocompatibility. Further implantation tests also indicated the fitness of the sonication-treated HUA as a scaffold for small-caliber tissue engineering vascular grafts. |
| 關聯: | POLYMERS-BASEL, v.13, n.11 |
| 顯示於類別: | [化妝品應用與管理系(所)] 期刊論文
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