Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering

doi:10.3390/polym13111699

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/34415

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34415

Title:	Sonication-Assisted Method for Decellularization of Human Umbilical Artery for Small-Caliber Vascular Tissue Engineering
Authors:	Lin, Chih-Hsun Hsia, Kai Su, Chih-Kuan Chen, Chien-Chin Yeh, Chang-Ching Ma, Hsu Lu, Jen-Her
Contributors:	Taipei Vet Gen Hosp, Dept Surg, Div Plast & Reconstruct Surg Natl Yang Ming Chiao Tung Univ, Sch Med, Dept Surg Chia Yi Christian Hosp, Ditmanson Med Fdn, Dept Pathol Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Taipei Vet Gen Hosp, Dept Obstet & Gynecol Natl Yang Ming Chiao Tung Univ, Dept Obstet & Gynecol Natl Taipei Univ Nursing & Hlth Sci, Dept Nurse Midwifery & Women Hlth Natl Yang Ming Chiao Tung Univ, Inst Clin Med Natl Def Med Ctr, Sch Med, Dept Surg Med & Pediat Taipei Med Univ Hosp, Sect Pediat Cardiol, Dept Pediat
Keywords:	sonication decellularized human umbilical artery ultrasonic decellularization extracellular matrix biocompatibility recellularization
Date:	2021
Issue Date:	2023-11-11 11:50:39 (UTC+8)
Publisher:	MDPI
Abstract:	Decellularized vascular grafts are useful for the construction of biological small-diameter tissue-engineered vascular grafts (<= 6 mm). Traditional chemical decellularization requires a long treatment time, which may damage the structure and alter the mechanical properties. Decellularization using sonication is expected to solve this problem. The aim of this study was to develop an effective decellularization method using ultrasound followed by washing. Different power values of sonication at 40 kHz were tested for 2, 4, and 8 h followed by a washing procedure. The efficacy of sonication of decellularized human umbilical artery (sDHUA) was evaluated via DNA content, histological staining, mechanical properties, and biocompatibility. The sDHUAs were further implanted into rats for up to 90 days and magnetic resonance angiography (MRA) was performed for the implanted grafts. The results demonstrated that treatment of human umbilical artery (HUA) by sonication at ultrasonic power of 204 W for 4 h followed by washing for 24 h in 2% SDS buffer could eliminate more than 90% of cells and retain similar mechanical properties of the HUA. Recellularization was assessed by scanning electron microscopy (SEM), which indicated that sDHUA provided niches for human umbilical vein endothelial cells (HUVECs) to reside, indicating in vitro cytocompatibility. Further implantation tests also indicated the fitness of the sonication-treated HUA as a scaffold for small-caliber tissue engineering vascular grafts.
Relation:	POLYMERS-BASEL, v.13, n.11
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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