Effects of Carissa carandas Linn. Fruit, Pulp, Leaf, and Seed on Oxidation, Inflammation, Tyrosinase, Matrix Metalloproteinase, Elastase, and Hyaluronidase Inhibition

doi:10.3390/antiox10091345

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/34371

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34371

Title:	Effects of Carissa carandas Linn. Fruit, Pulp, Leaf, and Seed on Oxidation, Inflammation, Tyrosinase, Matrix Metalloproteinase, Elastase, and Hyaluronidase Inhibition
Authors:	Neimkhum, Waranya Anuchapreeda, Songyot Lin, Wei-Chao Lue, Shang-Chian Lee, Kuan-Han Chaiyana, Wantida
Contributors:	Chiang Mai Univ, Fac Pharm, Dept Pharmaceut Sci Chiang Mai Univ, Fac Associated Med Sci, Dept Med Technol, Div Clin Microscopy Chiang Mai Univ, Res Ctr Pharmaceut Nanotechnol Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Chia Nan Univ Pharm & Sci, Inst Cosmet Sci Chia Nan Univ Pharm & Sci, Dept Pharm
Keywords:	Carissa carandas ursolic acid antioxidant anti-tyrosinase matrix metalloproteinases nuclear factor-kappa B interleukin human epidermal keratinocyte line
Date:	2021
Issue Date:	2023-11-11 11:47:56 (UTC+8)
Publisher:	MDPI
Abstract:	In this study, the potential of Carissa carandas Linn. as a natural anti-aging, antioxidant, and skin whitening agent was studied. Various parts of C. carandas, including fruit, leaf, seed, and pulp were sequentially extracted by maceration using n-hexane, ethyl acetate, and ethanol, respectively. High-performance liquid chromatography, Folin-Ciocalteu, and Dowd method were used to investigate their chemical compositions. The inhibitory activities of oxidation process, matrix metalloproteinases (MMPs), elastase, hyaluronidase, and tyrosinase were analyzed. Cytotoxicity was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide assay in a human epidermal keratinocyte line (HaCaT). The results exhibited that ethyl acetate could extract the most ursolic acid from C. carandas, while ethanol could extract the most phenolics and flavonoids. The leaf extract had the highest content of ursolic acid, phenolics, and flavonoids. The leaf extracted with ethyl acetate (AL) had the highest ursolic acid content (411.8 mg/g extract) and inhibited MMP-1, NF-kappa B, and tyrosinase activity the most. Ursolic acid has been proposed as a key component in these biological activities. Although several C. carandas extracts are beneficial to human skin, AL has been proposed for use in cosmetics and cosmeceuticals due to its superior anti-wrinkle, anti-inflammation, and whitening properties.
Relation:	ANTIOXIDANTS-BASEL, v.10, n.9, pp.1345
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles [Dept. of Pharmacy] Periodical Articles

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