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| 標題: | Chemical Constituents, Antioxidant, Anti-MMPs, and Anti-Hyaluronidase Activities of Thunbergia laurifolia Lindl. Leaf Extracts for Skin Aging and Skin Damage Prevention |
| 作者: | Chaiyana, Wantida
Chansakaow, Sunee
Intasai, Nutjeera
Kiattisin, Kanokwan
Lee, Kuan-Han
Lin, Wei-Chao
Lue, Shang-Chian
Leelapornpisid, Pimporn |
| 貢獻者: | Chiang Mai Univ, Dept Pharmaceut Sci, Fac Pharm
Chiang Mai Univ, Innovat Ctr Holist Hlth Nutraceut & Cosmeceut, Fac Pharm
Chiang Mai Univ, Fac Associated Med Sci, Dept Med Technol, Div Clin Microscopy
Chia Nan Univ Pharm & Sci, Dept Pharm
Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
Lin, Wei-Chao;Lue, Shang-Chian] Chia Nan Univ Pharm & Sci, Inst Cosmet Sci |
| 關鍵字: | Thunbergia laurifolia
matrix metalloproteinases
hyaluronidase
antioxidant
rosmarinic acid
cytotoxicity |
| 日期: | 2020 |
| 上傳時間: | 2022-11-18 11:23:54 (UTC+8) |
| 出版者: | Mdpi |
| 摘要: | This study aimed to investigate the potential usage of Thunbergia laurifolia Lindl. leaf extracts in the cosmetic industry. Matrix metalloproteinases (MMPs) and hyaluronidase inhibition of T. laurifolia leaf extracts, prepared using reflux extraction with deionized water (RE) and 80% v/v ethanol using Soxhlet's apparatus (SE), were determined. Rosmarinic acid, phenolics, and flavonoids contents were determined using high-performance liquid chromatography, Folin-Ciocalteu, and aluminum chloride colorimetric assays, respectively. Antioxidant activities were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and linoleic acid-thiocyanate assays. MMP-1 inhibition was investigated using enzymatic and fluorescent reactions, whereas MMP-2, MMP-9, and hyaluronidase inhibition were investigated using gel electrophoresis. Cytotoxicity on human fibroblast cell line was also investigated. The results demonstrated that SE contained significantly higher content of rosmarinic acid (5.62% +/- 0.01%) and flavonoids (417 +/- 25 mg of quercetin/g of extract) but RE contained a significantly higher phenolics content (181 +/- 1 mg of gallic acid/g of extract; p < 0.001). SE possessed higher lipid peroxidation inhibition but less DPPH center dot scavenging activity than RE. Both extracts possessed comparable hyaluronidase inhibition. SE was as potent an MMP-1 inhibitor as gallic acid (half maximal inhibitory concentration values were 12.0 +/- 0.3 and 8.9 +/- 0.4 mg/cm(3), respectively). SE showed significantly higher MMP-2 and MMP-9 inhibition than RE (p < 0.05). Therefore, SE is a promising natural anti-ageing ingredient rich in rosmarinic acid and flavonoids with antioxidant, anti-hyaluronidase, and potent MMPs inhibitory effects that could be applied in the cosmetic industry. |
| 關聯: | Molecules, v.25, n.8, pp.12 |
| 顯示於類別: | [化妝品應用與管理系(所)] 期刊論文
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