Chemical Constituents, Antioxidant, Anti-MMPs, and Anti-Hyaluronidase Activities of Thunbergia laurifolia Lindl. Leaf Extracts for Skin Aging and Skin Damage Prevention

doi:10.3390/molecules25081923

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 化妝品應用與管理系(所) > 期刊論文 > Item 310902800/34100

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標題:	Chemical Constituents, Antioxidant, Anti-MMPs, and Anti-Hyaluronidase Activities of Thunbergia laurifolia Lindl. Leaf Extracts for Skin Aging and Skin Damage Prevention
作者:	Chaiyana, Wantida Chansakaow, Sunee Intasai, Nutjeera Kiattisin, Kanokwan Lee, Kuan-Han Lin, Wei-Chao Lue, Shang-Chian Leelapornpisid, Pimporn
貢獻者:	Chiang Mai Univ, Dept Pharmaceut Sci, Fac Pharm Chiang Mai Univ, Innovat Ctr Holist Hlth Nutraceut & Cosmeceut, Fac Pharm Chiang Mai Univ, Fac Associated Med Sci, Dept Med Technol, Div Clin Microscopy Chia Nan Univ Pharm & Sci, Dept Pharm Chia Nan Univ Pharm & Sci, Dept Cosmet Sci Lin, Wei-Chao;Lue, Shang-Chian] Chia Nan Univ Pharm & Sci, Inst Cosmet Sci
關鍵字:	Thunbergia laurifolia matrix metalloproteinases hyaluronidase antioxidant rosmarinic acid cytotoxicity
日期:	2020
上傳時間:	2022-11-18 11:23:54 (UTC+8)
出版者:	Mdpi
摘要:	This study aimed to investigate the potential usage of Thunbergia laurifolia Lindl. leaf extracts in the cosmetic industry. Matrix metalloproteinases (MMPs) and hyaluronidase inhibition of T. laurifolia leaf extracts, prepared using reflux extraction with deionized water (RE) and 80% v/v ethanol using Soxhlet's apparatus (SE), were determined. Rosmarinic acid, phenolics, and flavonoids contents were determined using high-performance liquid chromatography, Folin-Ciocalteu, and aluminum chloride colorimetric assays, respectively. Antioxidant activities were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and linoleic acid-thiocyanate assays. MMP-1 inhibition was investigated using enzymatic and fluorescent reactions, whereas MMP-2, MMP-9, and hyaluronidase inhibition were investigated using gel electrophoresis. Cytotoxicity on human fibroblast cell line was also investigated. The results demonstrated that SE contained significantly higher content of rosmarinic acid (5.62% +/- 0.01%) and flavonoids (417 +/- 25 mg of quercetin/g of extract) but RE contained a significantly higher phenolics content (181 +/- 1 mg of gallic acid/g of extract; p < 0.001). SE possessed higher lipid peroxidation inhibition but less DPPH center dot scavenging activity than RE. Both extracts possessed comparable hyaluronidase inhibition. SE was as potent an MMP-1 inhibitor as gallic acid (half maximal inhibitory concentration values were 12.0 +/- 0.3 and 8.9 +/- 0.4 mg/cm(3), respectively). SE showed significantly higher MMP-2 and MMP-9 inhibition than RE (p < 0.05). Therefore, SE is a promising natural anti-ageing ingredient rich in rosmarinic acid and flavonoids with antioxidant, anti-hyaluronidase, and potent MMPs inhibitory effects that could be applied in the cosmetic industry.
關聯:	Molecules, v.25, n.8, pp.12
显示于类别:	[化妝品應用與管理系(所)] 期刊論文

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