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| 標題: | Anti-diabetic effects of Grifola frondosa bioactive compound and its related molecular signaling pathways in palmitate-induced C2C12 cells |
| 作者: | Wu, Shu-Jing
Tung, Yi-Jou
Ng, Lean-Teik |
| 貢獻者: | Chia Nan Univ Pharm & Sci, Dept Nutr Hlth
Natl Taiwan Univ, Dept Agr Chem |
| 關鍵字: | Ergosterol peroxide
C2C12 cells
PI3K
Akt
MAPKs
GLUT-4 |
| 日期: | 2020 |
| 上傳時間: | 2022-11-18 11:22:54 (UTC+8) |
| 出版者: | Elsevier Ireland Ltd |
| 摘要: | Ethnopharmacological relevance: Grifola frondosa (GF), a high value medicinal mushroom, is popularly consumed as traditional medicines and health foods in China and Japan. It is a herbal medicine traditionally used for treating inflammation, cancer and diabetes. Aim of the study: This study aimed to examine the anti-diabetic effects of a GF bioactive compound ergosterol peroxide (EPO), and its mechanism(s) of action in palmitate (PA)-induced C2C12 cells. Materials and methods: EPO was isolated and purified from GF fruiting bodies, and used to test for anti-diabetic activity in PA-induced murine C2C12 skeletal muscle cells through measuring glucose uptake, intracellular ROS production, and expressions of MAPKs, IRS-1, PI3K, Akt and GLUT-4 proteins. Results: EPO significantly up-regulated glucose absorption and increased cell growth. At 5 mu M, EPO significantly enhanced glucose uptake and decreased ROS formation, as well as up-regulated the expression of IRS-1, p-IRS-1, PI3K, Akt, p-Akt, and GLUT-4 proteins in PA-induced cells, while their p-JNK and p-p38 expression were downregulated. GLUT-4 siRNA treatment effectively down-regulated the EPO-induced absorption of glucose and inhibited the expression of GLUT-4. Conclusion: These results suggest that the anti-diabetic effect of GF was from its bioactive compound EPO through the inhibition of ROS production, up-regulation of glucose absorption, and modulation of PI3K/Akt, MAPKs and GLUT-4 signaling transduction pathways. |
| 關聯: | Journal of Ethnopharmacology, v.260, pp.9 |
| 顯示於類別: | [保健營養系(所) ] 期刊論文
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