Anti-diabetic effects of Grifola frondosa bioactive compound and its related molecular signaling pathways in palmitate-induced C2C12 cells

doi:10.1016/j.jep.2020.112962

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/34075

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/34075

Title:	Anti-diabetic effects of Grifola frondosa bioactive compound and its related molecular signaling pathways in palmitate-induced C2C12 cells
Authors:	Wu, Shu-Jing Tung, Yi-Jou Ng, Lean-Teik
Contributors:	Chia Nan Univ Pharm & Sci, Dept Nutr Hlth Natl Taiwan Univ, Dept Agr Chem
Keywords:	Ergosterol peroxide C2C12 cells PI3K Akt MAPKs GLUT-4
Date:	2020
Issue Date:	2022-11-18 11:22:54 (UTC+8)
Publisher:	Elsevier Ireland Ltd
Abstract:	Ethnopharmacological relevance: Grifola frondosa (GF), a high value medicinal mushroom, is popularly consumed as traditional medicines and health foods in China and Japan. It is a herbal medicine traditionally used for treating inflammation, cancer and diabetes. Aim of the study: This study aimed to examine the anti-diabetic effects of a GF bioactive compound ergosterol peroxide (EPO), and its mechanism(s) of action in palmitate (PA)-induced C2C12 cells. Materials and methods: EPO was isolated and purified from GF fruiting bodies, and used to test for anti-diabetic activity in PA-induced murine C2C12 skeletal muscle cells through measuring glucose uptake, intracellular ROS production, and expressions of MAPKs, IRS-1, PI3K, Akt and GLUT-4 proteins. Results: EPO significantly up-regulated glucose absorption and increased cell growth. At 5 mu M, EPO significantly enhanced glucose uptake and decreased ROS formation, as well as up-regulated the expression of IRS-1, p-IRS-1, PI3K, Akt, p-Akt, and GLUT-4 proteins in PA-induced cells, while their p-JNK and p-p38 expression were downregulated. GLUT-4 siRNA treatment effectively down-regulated the EPO-induced absorption of glucose and inhibited the expression of GLUT-4. Conclusion: These results suggest that the anti-diabetic effect of GF was from its bioactive compound EPO through the inhibition of ROS production, up-regulation of glucose absorption, and modulation of PI3K/Akt, MAPKs and GLUT-4 signaling transduction pathways.
Relation:	Journal of Ethnopharmacology, v.260, pp.9
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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