English  |  正體中文  |  简体中文  |  Items with full text/Total items : 17776/20117 (88%)
Visitors : 12171849      Online Users : 211
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: http://ir.cnu.edu.tw/handle/310902800/32616

    標題: Modulation of parietal cytokine and chemokine gene profiles by mesenchymal stem cell as a basis for neurotrauma recovery
    作者: Lin, Cheng-Hsien
    Lin, Willie
    Su, Yu-Chin
    Hsuan, Yogi Cheng-Yo
    Chen, Yu-Chien
    Chang, Ching-Ping
    Willy Chou(周偉倪)
    Lin, Kao-Chang
    貢獻者: Mackay Med Coll, Dept Med
    Meridigen Biotech Co Ltd
    Chi Mei Med Ctr, Dept Med Res
    Chi Mei Med Ctr, Dept Phys Med & Rehabil
    Chia Nan Univ Pharm & Sci, Dept Recreat & Healthcare Management
    Chi Mei Med Ctr, Dept Neurol
    關鍵字: Mesenchymal stem cells
    Traumatic brain injury
    日期: 2019-12
    上傳時間: 2020-07-29 13:52:15 (UTC+8)
    摘要: Background & purpose: Following traumatic brain injury (TBI), primary mechanical injury to the brain may cause blood-brain-barrier damage followed by secondary injury, ultimately culminating in cell death. We aimed to test whether one injection of mesenchymal stem cells (MSC) derived from the human umbilical cord can modulate brain cytokine and chemokine gene profiles and attenuate neurological injury in rats with TBI. Methods: One-day post-TBI, the injured rats were treated with one injection of MSC (4 x 10(6)/rat, i.v.). Three days later, immediately after assessment of neurobehavioral function, animals were sacrificed for analysis of neurological injury (evidenced by both brain contusion volume and neurological deficits) and parietal genes encoding 84 cytokines and chemokines in the injured brain by qPCR methods. Results: Three days post-TBI, rats displayed both neurological injury and upgrade of 11 parietal genes in the ipsilateral brain. One set of 8 parietal genes (e.g., chemokine [C-X-C motif] ligand 12, platelet factor 4, interleukin-7, chemokine [C-C motif] ligand (CCL)19, CCL 22, secreted phosphoprotein 1, pro-platelet basic protein 1, and CCL 2) differentially upgraded by TBI was related to pro-inflammatory and/or neurodegenerative processes. Another set of 3 parietal genes up-graded by TBI (e.g., glucose-6-phosphate isomerase, bone morphogenetic protein (BMP) 2, and BMP 4) was related to anti-inflammatory/neuroregenerative events. Administration of MSC attenuated neurological injury, down-regulated these 8 parietal pro-inflammatory genes, and up-regulated these 3 parietal anti-inflammatory genes in the rats with TBI. Conclusion: Our data suggest that modulation of parietal cytokines and chemokines gene profiles by MSC as a basis for neurotrauma recovery. Copyright (C) 2019, Formosan Medical Association. Published by Elsevier Taiwan LLC.
    關聯: Journal of the Formosan Medical Association, v.118, n.12, pp.1661-1673
    Appears in Collections:[休閒保健管理系(所)] 期刊論文

    Files in This Item:

    File Description SizeFormat
    10.1016-j.jfma.2019.01.008.pdf3322KbAdobe PDF116View/Open

    All items in CNU IR are protected by copyright, with all rights reserved.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback