Modulation of parietal cytokine and chemokine gene profiles by mesenchymal stem cell as a basis for neurotrauma recovery

doi:10.1016/j.jfma.2019.01.008

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標題:	Modulation of parietal cytokine and chemokine gene profiles by mesenchymal stem cell as a basis for neurotrauma recovery
作者:	Lin, Cheng-Hsien Lin, Willie Su, Yu-Chin Hsuan, Yogi Cheng-Yo Chen, Yu-Chien Chang, Ching-Ping Willy Chou(周偉倪) Lin, Kao-Chang
貢獻者:	Mackay Med Coll, Dept Med Meridigen Biotech Co Ltd Chi Mei Med Ctr, Dept Med Res Chi Mei Med Ctr, Dept Phys Med & Rehabil Chia Nan Univ Pharm & Sci, Dept Recreat & Healthcare Management Chi Mei Med Ctr, Dept Neurol
關鍵字:	Mesenchymal stem cells Traumatic brain injury Inflammation Chemokines Cytokines
日期:	2019-12
上傳時間:	2020-07-29 13:52:15 (UTC+8)
出版者:	ELSEVIER TAIWAN
摘要:	Background & purpose: Following traumatic brain injury (TBI), primary mechanical injury to the brain may cause blood-brain-barrier damage followed by secondary injury, ultimately culminating in cell death. We aimed to test whether one injection of mesenchymal stem cells (MSC) derived from the human umbilical cord can modulate brain cytokine and chemokine gene profiles and attenuate neurological injury in rats with TBI. Methods: One-day post-TBI, the injured rats were treated with one injection of MSC (4 x 10(6)/rat, i.v.). Three days later, immediately after assessment of neurobehavioral function, animals were sacrificed for analysis of neurological injury (evidenced by both brain contusion volume and neurological deficits) and parietal genes encoding 84 cytokines and chemokines in the injured brain by qPCR methods. Results: Three days post-TBI, rats displayed both neurological injury and upgrade of 11 parietal genes in the ipsilateral brain. One set of 8 parietal genes (e.g., chemokine [C-X-C motif] ligand 12, platelet factor 4, interleukin-7, chemokine [C-C motif] ligand (CCL)19, CCL 22, secreted phosphoprotein 1, pro-platelet basic protein 1, and CCL 2) differentially upgraded by TBI was related to pro-inflammatory and/or neurodegenerative processes. Another set of 3 parietal genes up-graded by TBI (e.g., glucose-6-phosphate isomerase, bone morphogenetic protein (BMP) 2, and BMP 4) was related to anti-inflammatory/neuroregenerative events. Administration of MSC attenuated neurological injury, down-regulated these 8 parietal pro-inflammatory genes, and up-regulated these 3 parietal anti-inflammatory genes in the rats with TBI. Conclusion: Our data suggest that modulation of parietal cytokines and chemokines gene profiles by MSC as a basis for neurotrauma recovery. Copyright (C) 2019, Formosan Medical Association. Published by Elsevier Taiwan LLC.
關聯:	Journal of the Formosan Medical Association, v.118, n.12, pp.1661-1673
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