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| 標題: | Angiotensin 1-7 modulates electrophysiological characteristics and calcium homoeostasis in pulmonary veins cardiomyocytes via MAS/PI3K/eNOS signalling pathway |
| 作者: | Lu, Yen-Yu
Wu, Wen-Shiann
Lin, Yung-Kuo
Cheng, Chen-Chuan
Chen, Yao-Chang
Chen, Shih-Ann
Chen, Yi-Jen |
| 貢獻者: | Sijhih Cathay Gen Hosp, Div Cardiol, Dept Internal Med
Fu Jen Catholic Univ, Sch Med
Chi Mei Med Ctr, Dept Cardiol
Chia Nan Univ Pharm & Sci, Dept Pharm
Taipei Med Univ, Div Cardiovasc Med, Dept Internal Med, Wan Fang Hosp
Taipei Med Univ, Div Cardiol, Dept Internal Med, Sch Med,Coll Med
Natl Def Med Ctr, Dept Biomed Engn
Natl Yang Ming Univ, Sch Med, Div Cardiol
Natl Yang Ming Univ, Vet Gen Hosp Taipei, Cardiovasc Res Ctr
Taipei Med Univ, Grad Inst Clin Med, Coll Med |
| 關鍵字: | angiotensin-(1-7)
calcium homoeostasis
electrophysiology
pulmonary vein |
| 日期: | 2018-01 |
| 上傳時間: | 2019-11-15 15:48:52 (UTC+8) |
| 出版者: | WILEY |
| 摘要: | BackgroundAtrial fibrillation (AF) is the most common sustained arrhythmia, and pulmonary veins (PVs) play a critical role in triggering AF. Angiotensin (Ang)-(1-7) regulates calcium (Ca2+) homoeostasis and also plays a critical role in cardiovascular pathophysiology. However, the role of Ang-(1-7) in PV arrhythmogenesis remains unclear. Materials and methodsConventional microelectrodes, whole-cell patch-clamp and the fluo-3 fluorimetric ratio technique were used to record ionic currents and intracellular Ca2+ in isolated rabbit PV preparations and in single isolated PV cardiomyocytes, before and after administration of Ang-(1-7). ResultsAng (1-7) concentration dependently (0.1, 1, 10 and 100 nmol/L) decreased PV spontaneous electrical activity. Ang-(1-7) (100 nmol/L) decreased the late sodium (Na+), L-type Ca2+ and Na+-Ca2+ exchanger currents, but did not affect the voltage-dependent Na+ current in PV cardiomyocytes. In addition, Ang-(1-7) decreased intracellular Ca2+ transient and sarcoplasmic reticulum Ca2+ content in PV cardiomyocytes. A779 (a Mas receptor blocker, 3 mol/L), L-NAME (a NO synthesis inhibitor, 100 mol/L) or wortmannin (a specific PI3K inhibitor, 10 nmol/L) attenuated the effects of Ang-(1-7) (100 nmol/L) on PV spontaneous electric activity. ConclusionAng-(1-7) regulates PV electrophysiological characteristics and Ca2+ homoeostasis via Mas/PI3K/eNOS signalling pathway. |
| link: | https://doi.org/10.1111/eci.12854 |
| 關聯: | Molecular Carcinogenesis, v.48, n.1, pp.UNSP e12854 |
| 顯示於類別: | [藥學系(所)] 期刊論文
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