Angiotensin 1-7 modulates electrophysiological characteristics and calcium homoeostasis in pulmonary veins cardiomyocytes via MAS/PI3K/eNOS signalling pathway

doi:10.1111/eci.12854

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Title:	Angiotensin 1-7 modulates electrophysiological characteristics and calcium homoeostasis in pulmonary veins cardiomyocytes via MAS/PI3K/eNOS signalling pathway
Authors:	Lu, Yen-Yu Wu, Wen-Shiann Lin, Yung-Kuo Cheng, Chen-Chuan Chen, Yao-Chang Chen, Shih-Ann Chen, Yi-Jen
Contributors:	Sijhih Cathay Gen Hosp, Div Cardiol, Dept Internal Med Fu Jen Catholic Univ, Sch Med Chi Mei Med Ctr, Dept Cardiol Chia Nan Univ Pharm & Sci, Dept Pharm Taipei Med Univ, Div Cardiovasc Med, Dept Internal Med, Wan Fang Hosp Taipei Med Univ, Div Cardiol, Dept Internal Med, Sch Med,Coll Med Natl Def Med Ctr, Dept Biomed Engn Natl Yang Ming Univ, Sch Med, Div Cardiol Natl Yang Ming Univ, Vet Gen Hosp Taipei, Cardiovasc Res Ctr Taipei Med Univ, Grad Inst Clin Med, Coll Med
Keywords:	angiotensin-(1-7) calcium homoeostasis electrophysiology pulmonary vein
Date:	2018-01
Issue Date:	2019-11-15 15:48:52 (UTC+8)
Publisher:	WILEY
Abstract:	BackgroundAtrial fibrillation (AF) is the most common sustained arrhythmia, and pulmonary veins (PVs) play a critical role in triggering AF. Angiotensin (Ang)-(1-7) regulates calcium (Ca2+) homoeostasis and also plays a critical role in cardiovascular pathophysiology. However, the role of Ang-(1-7) in PV arrhythmogenesis remains unclear. Materials and methodsConventional microelectrodes, whole-cell patch-clamp and the fluo-3 fluorimetric ratio technique were used to record ionic currents and intracellular Ca2+ in isolated rabbit PV preparations and in single isolated PV cardiomyocytes, before and after administration of Ang-(1-7). ResultsAng (1-7) concentration dependently (0.1, 1, 10 and 100 nmol/L) decreased PV spontaneous electrical activity. Ang-(1-7) (100 nmol/L) decreased the late sodium (Na+), L-type Ca2+ and Na+-Ca2+ exchanger currents, but did not affect the voltage-dependent Na+ current in PV cardiomyocytes. In addition, Ang-(1-7) decreased intracellular Ca2+ transient and sarcoplasmic reticulum Ca2+ content in PV cardiomyocytes. A779 (a Mas receptor blocker, 3 mol/L), L-NAME (a NO synthesis inhibitor, 100 mol/L) or wortmannin (a specific PI3K inhibitor, 10 nmol/L) attenuated the effects of Ang-(1-7) (100 nmol/L) on PV spontaneous electric activity. ConclusionAng-(1-7) regulates PV electrophysiological characteristics and Ca2+ homoeostasis via Mas/PI3K/eNOS signalling pathway.
???metadata.dc.relation.uri???:	https://doi.org/10.1111/eci.12854
Relation:	Molecular Carcinogenesis, v.48, n.1, pp.UNSP e12854
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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