Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy

doi:10.1111/jop.12593

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標題:	Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy
作者:	Lin, Mei-Huei Liu, Young-Chau Liu, Shyun-Yeu Chen, Fang-Chi Yang, Pei-Jung Li, Guan-Hua Liu, Pang-Yen Yen, Ching-Yu
貢獻者:	Chia Nan Univ Pharm, Dept Biotechnol Chi Mei Med Ctr, Dept Med Res Shu Te Univ, Div Nat Sci, Coll Liberal Educ Taipei Med Univ, Dept Dent Natl Def Med Ctr, Dept Dent Chi Mei Med Ctr, Oral & Maxillofacial Surg Sect
關鍵字:	areca nut autophagy benzyl alcohol clathrin dynamin dynasore endocytosis
日期:	2018-01
上傳時間:	2019-11-15 15:48:50 (UTC+8)
出版者:	WILEY
摘要:	BackgroundWe identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. MethodsWe used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. ResultsBoth benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. ConclusionsThese results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.
link:	http://dx.doi.org/10.1111/jop.12593
關聯:	European Journal of Clinical Investigation, v.47, n.1, pp.25-31
显示于类别:	[生物科技系(所)] 期刊論文

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