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    Title: Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy
    Authors: Lin, Mei-Huei
    Liu, Young-Chau
    Liu, Shyun-Yeu
    Chen, Fang-Chi
    Yang, Pei-Jung
    Li, Guan-Hua
    Liu, Pang-Yen
    Yen, Ching-Yu
    Contributors: Chia Nan Univ Pharm, Dept Biotechnol
    Chi Mei Med Ctr, Dept Med Res
    Shu Te Univ, Div Nat Sci, Coll Liberal Educ
    Taipei Med Univ, Dept Dent
    Natl Def Med Ctr, Dept Dent
    Chi Mei Med Ctr, Oral & Maxillofacial Surg Sect
    Keywords: areca nut
    benzyl alcohol
    Date: 2018-01
    Issue Date: 2019-11-15 15:48:50 (UTC+8)
    Publisher: WILEY
    Abstract: BackgroundWe identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. MethodsWe used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. ResultsBoth benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. ConclusionsThese results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.
    Relation: European Journal of Clinical Investigation, v.47, n.1, pp.25-31
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Periodical Articles

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