Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy

doi:10.1111/jop.12593

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Periodical Articles > Item 310902800/32304

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/32304

Title:	Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy
Authors:	Lin, Mei-Huei Liu, Young-Chau Liu, Shyun-Yeu Chen, Fang-Chi Yang, Pei-Jung Li, Guan-Hua Liu, Pang-Yen Yen, Ching-Yu
Contributors:	Chia Nan Univ Pharm, Dept Biotechnol Chi Mei Med Ctr, Dept Med Res Shu Te Univ, Div Nat Sci, Coll Liberal Educ Taipei Med Univ, Dept Dent Natl Def Med Ctr, Dept Dent Chi Mei Med Ctr, Oral & Maxillofacial Surg Sect
Keywords:	areca nut autophagy benzyl alcohol clathrin dynamin dynasore endocytosis
Date:	2018-01
Issue Date:	2019-11-15 15:48:50 (UTC+8)
Publisher:	WILEY
Abstract:	BackgroundWe identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. MethodsWe used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. ResultsBoth benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. ConclusionsThese results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.
???metadata.dc.relation.uri???:	http://dx.doi.org/10.1111/jop.12593
Relation:	European Journal of Clinical Investigation, v.47, n.1, pp.25-31
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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