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| 標題: | Heat Shock Protein 70 (HSP70) Reduces Hepatic Inflammatory and Oxidative Damage in a Rat Model of Liver lschemia/Reperfusion Injury with Hyperbaric Oxygen Preconditioning |
| 作者: | Wu, Hsing-Hsien
Huang, Chien-Cheng
Chang, Ching-Ping
Lin, Mao-Tsun
Niu, Ko-Chi
Tian, Yu-Feng |
| 貢獻者: | Tainan Municipal Hosp, Dept Surg
Chi Mei Med Ctr, Dept Emergency Med
Southern Taiwan Univ Sci & Technol, Dept Senior Serv
Natl Cheng Kung Univ, Coll Med, Dept Environm & Occupat Hlth
Chi Mei Med Ctr, Dept Geriatr & Geront
Chi Mei Med Ctr, Dept Occupat Med
Chi Mei Med Ctr, Dept Med Res
Chi Mei Med Ctr, Dept Hyperbar Oxygen Med
Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr
Chi Mei Med Ctr, Dept Surg, Div Colorectal Surg |
| 關鍵字: | HSP70 Heat-Shock Proteins
Hyperbaric Oxygenation
Liver Failure, Acute
Reperfusion Injury |
| 日期: | 2018-11-12 |
| 上傳時間: | 2019-11-15 15:45:48 (UTC+8) |
| 出版者: | INT SCIENTIFIC INFORMATION, INC |
| 摘要: | Background: Several clinical conditions can cause hepatic ischemia/reperfusion (I/R) injury. This study aimed to determine the mechanism of the protective effect of hyperbaric oxygen preconditioning (HBO2P) on hepatic ischemia/reperfusion (I/R) injury in a rat model, and to investigate the effects on HBO2P and I/R injury of blocking HSP70 using antibody (Ab) pretreatment. Material/Methods: Male Sprague-Dawley rats underwent HBO2P for 60 min at 2.0 atmosphere absolute (ATA) pressure for five consecutive days before surgical hepatic I/R injury, performed by clamping the portal vein and hepatic lobe. Four groups studied included: the non-HBO2P+ non-I/R group, which underwent sham surgery (N=10); the non-HBO2P + I/R group (N=10); the HBO2P + I/R group (N=10); and the HBO2P + HSP70-Ab + I/R group (N=10) received one dose of HSP70 antibody one day before hepatic I/R injury. Serum lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), and hepatic malondialdehyde (MDA) and myeloperoxidase (MPO) were measured biochemically. Rat liver tissues were examined histologically. Results: In rats with hepatic I/R injury without HSP70 antibody pre-treatment, HBO2P significantly reduced hepatic injury and levels of LDH, AST, ALT, TNF-alpha, IL-6, MDA, and MPO levels; in comparison, the group pre-treated with an antibody to inhibit HSP70 (the HBO2P + HSP70-Ab + I/R group) showed significant reversal of the beneficial effects of HBO2P on hepatic I/R injury (p<0.05). Conclusions: In a rat model of hepatic I/R injury with HBO2P, HSP70 reduced hepatic inflammatory and oxidative damage. |
| 關聯: | International Journal of Medical Sciences, v.24, pp.8096-8104 |
| 顯示於類別: | [保健營養系(所) ] 期刊論文
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