Heat Shock Protein 70 (HSP70) Reduces Hepatic Inflammatory and Oxidative Damage in a Rat Model of Liver lschemia/Reperfusion Injury with Hyperbaric Oxygen Preconditioning

doi:10.12659/MSM.911641

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Title:	Heat Shock Protein 70 (HSP70) Reduces Hepatic Inflammatory and Oxidative Damage in a Rat Model of Liver lschemia/Reperfusion Injury with Hyperbaric Oxygen Preconditioning
Authors:	Wu, Hsing-Hsien Huang, Chien-Cheng Chang, Ching-Ping Lin, Mao-Tsun Niu, Ko-Chi Tian, Yu-Feng
Contributors:	Tainan Municipal Hosp, Dept Surg Chi Mei Med Ctr, Dept Emergency Med Southern Taiwan Univ Sci & Technol, Dept Senior Serv Natl Cheng Kung Univ, Coll Med, Dept Environm & Occupat Hlth Chi Mei Med Ctr, Dept Geriatr & Geront Chi Mei Med Ctr, Dept Occupat Med Chi Mei Med Ctr, Dept Med Res Chi Mei Med Ctr, Dept Hyperbar Oxygen Med Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Chi Mei Med Ctr, Dept Surg, Div Colorectal Surg
Keywords:	HSP70 Heat-Shock Proteins Hyperbaric Oxygenation Liver Failure, Acute Reperfusion Injury
Date:	2018-11-12
Issue Date:	2019-11-15 15:45:48 (UTC+8)
Publisher:	INT SCIENTIFIC INFORMATION, INC
Abstract:	Background: Several clinical conditions can cause hepatic ischemia/reperfusion (I/R) injury. This study aimed to determine the mechanism of the protective effect of hyperbaric oxygen preconditioning (HBO2P) on hepatic ischemia/reperfusion (I/R) injury in a rat model, and to investigate the effects on HBO2P and I/R injury of blocking HSP70 using antibody (Ab) pretreatment. Material/Methods: Male Sprague-Dawley rats underwent HBO2P for 60 min at 2.0 atmosphere absolute (ATA) pressure for five consecutive days before surgical hepatic I/R injury, performed by clamping the portal vein and hepatic lobe. Four groups studied included: the non-HBO2P+ non-I/R group, which underwent sham surgery (N=10); the non-HBO2P + I/R group (N=10); the HBO2P + I/R group (N=10); and the HBO2P + HSP70-Ab + I/R group (N=10) received one dose of HSP70 antibody one day before hepatic I/R injury. Serum lactate dehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and pro-inflammatory cytokines, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6), and hepatic malondialdehyde (MDA) and myeloperoxidase (MPO) were measured biochemically. Rat liver tissues were examined histologically. Results: In rats with hepatic I/R injury without HSP70 antibody pre-treatment, HBO2P significantly reduced hepatic injury and levels of LDH, AST, ALT, TNF-alpha, IL-6, MDA, and MPO levels; in comparison, the group pre-treated with an antibody to inhibit HSP70 (the HBO2P + HSP70-Ab + I/R group) showed significant reversal of the beneficial effects of HBO2P on hepatic I/R injury (p<0.05). Conclusions: In a rat model of hepatic I/R injury with HBO2P, HSP70 reduced hepatic inflammatory and oxidative damage.
Relation:	International Journal of Medical Sciences, v.24, pp.8096-8104
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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