A novel indication of platonin, a therapeutic immunomodulating medicine, on neuroprotection against ischemic stroke in mice

doi:10.1038/srep42277

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Title:	A novel indication of platonin, a therapeutic immunomodulating medicine, on neuroprotection against ischemic stroke in mice
Authors:	Sheu, Joen-Rong Chen, Zhih-Cherng Jayakumar, Thanasekaran Chou, Duen-Suey Yen, Ting-Lin Lee, Hsing-Ni Pan, Szu-Han Hsia, Chih-Hsuan Yang, Chih-Hao Hsieh, Cheng-Ying
Contributors:	Taipei Med Univ, Grad Inst Med Sci, Coll Med Taipei Med Univ, Dept Pharmacol, Coll Med Chi Mei Med Ctr, Dept Cardiol Chia Nan Univ Pharm & Sci, Dept Pharm
Keywords:	Cerebral-Ischemia In-Vivo Antithrombotic Therapy Photosensitizing Dye Murine Macrophages Reperfusion Injury Oxidative Stress Focal Ischemia Rats Cells
Date:	2017-02-06
Issue Date:	2018-11-30 15:56:49 (UTC+8)
Publisher:	Nature Publishing Group
Abstract:	Thrombosis and stroke are major causes of disability and death worldwide. However, the regular antithrombotic drugs may have unsatisfactory results and side effects. Platonin, a cyanine photosensitizing dye, has been used to treat trauma, ulcers and some acute inflammation. Here, we explored the neuroprotective effects of platonin against middle cerebral artery occlusion (MCAO)-induced ischemic stroke in mice. Platonin(200 mu g/kg) substantially reduced cerebral infarct volume, brain edema, neuronal cell death and neurological deficit scores, and improved the MCAO-reduced locomotor activity and rotarod performance. Platonin(5-10 mu M) potently inhibited platelet aggregation and c-Jun NH2-terminal kinase (JNK) phosphorylation in collagen-activated platelets. The antiaggregation effect did not affect bleeding time but increased occlusion time in platonin(100 and 200 mu g/kg)-treated mice. Platonin(2-10 mu M) was potent in diminishing collagen-and Fenton reaction-induced (OH)-O-center dot formation. Platonin(5-10 mu M) also suppressed the expression of nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, interleukin-1 beta, and JNK phosphorylation in lipopolysaccharide-stimulated macrophages. MCAO-induced expression of 3-nitrotyrosine and Iba1 was apparently attenuated in platonin(200 mu g/kg)-treated mice. In conclusion, platonin exhibited remarkable neuroprotective properties against MCAO-induced ischemia in a mouse model through its antiaggregation, antiinflammatory and antiradical properties. The observed therapeutic efficacy of platonin may consider being a novel medcine against ischemic stroke.
Relation:	Scientific Reports, v.7, pp.42277
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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