Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/31730
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 17775/20116 (88%)
造访人次 : 10209221      在线人数 : 469
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧:
  • 您可在西文检索词汇前后加上"双引号",以获取较精准的检索结果
  • 若欲以作者姓名搜寻,建议至进阶搜寻限定作者字段,可获得较完整数据
  • 进阶搜寻


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.cnu.edu.tw/handle/310902800/31730


    標題: Production and Anti-Melanoma Activity of Methoxyisoflavones from the Biotransformation of Genistein by Two Recombinant Escherichia coli Strains
    作者: Chiang, Chien-Min
    Chang, Yu-Jhe
    Wu, Jiumn-Yih
    Chang, Te-Sheng
    貢獻者: Chia Nan Univ Pharm & Sci, Dept Biotechnol
    Natl Univ Tainan, Dept Biol Sci & Technol
    I Shou Univ, Inst Biotechnol & Chem Engn
    Natl Quemoy Univ, Ctr Gen Educ
    關鍵字: genistein
    melanoma
    methoxyisoflavone
    methyltransferase
    tyrosinase
    日期: 2017-01
    上傳時間: 2018-11-30 15:54:32 (UTC+8)
    出版者: Mdpi Ag
    摘要: Biotransformation of the soy isoflavone genistein by sequential 3-hydroxylation using recombinant Escherichia coli expressing tyrosinase from Bacillus megaterium and then methylation using another recombinant E. coli expressing O-methyltransferase from Streptomyces peucetius was conducted. The results showed that two metabolites were produced from the biotransformation, identified as 5,7,4-trihydroxy-3-methoxyisoflavone and 5,7,3-trihydroxy-4-methoxyisoflavone, respectively, based on their mass and nuclear magnetic resonance spectral data. 5,7,4-Trihydroxy-3-methoxyisoflavone showed potent antiproliferative activity toward mouse B16 melanoma cells with an IC50 value of 68.8 M. In contrast, the compound did not show any cytotoxicity toward mouse normal fibroblast cells, even at 350 M concentration. The results of the present study offer insight on the production of both 5,7,4-trihydroxy-3-methoxyisoflavone and 5,7,3-trihydroxy-4-methoxyisoflavone by two recombinant E. coli strains and the potential anti-melanoma applications of 5,7,4-trihydroxy-3-methoxyisoflavone.
    關聯: Molecules, v.22, n.1, UNSP 87
    显示于类别:[生物科技系(所)] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    10.3390-molecules22010087.pdf679KbAdobe PDF0检视/开启
    index.html0KbHTML355检视/开启


    在CNU IR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈