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    Title: Production and Anti-Melanoma Activity of Methoxyisoflavones from the Biotransformation of Genistein by Two Recombinant Escherichia coli Strains
    Authors: Chiang, Chien-Min
    Chang, Yu-Jhe
    Wu, Jiumn-Yih
    Chang, Te-Sheng
    Contributors: Chia Nan Univ Pharm & Sci, Dept Biotechnol
    Natl Univ Tainan, Dept Biol Sci & Technol
    I Shou Univ, Inst Biotechnol & Chem Engn
    Natl Quemoy Univ, Ctr Gen Educ
    Keywords: genistein
    Date: 2017-01
    Issue Date: 2018-11-30 15:54:32 (UTC+8)
    Publisher: Mdpi Ag
    Abstract: Biotransformation of the soy isoflavone genistein by sequential 3-hydroxylation using recombinant Escherichia coli expressing tyrosinase from Bacillus megaterium and then methylation using another recombinant E. coli expressing O-methyltransferase from Streptomyces peucetius was conducted. The results showed that two metabolites were produced from the biotransformation, identified as 5,7,4-trihydroxy-3-methoxyisoflavone and 5,7,3-trihydroxy-4-methoxyisoflavone, respectively, based on their mass and nuclear magnetic resonance spectral data. 5,7,4-Trihydroxy-3-methoxyisoflavone showed potent antiproliferative activity toward mouse B16 melanoma cells with an IC50 value of 68.8 M. In contrast, the compound did not show any cytotoxicity toward mouse normal fibroblast cells, even at 350 M concentration. The results of the present study offer insight on the production of both 5,7,4-trihydroxy-3-methoxyisoflavone and 5,7,3-trihydroxy-4-methoxyisoflavone by two recombinant E. coli strains and the potential anti-melanoma applications of 5,7,4-trihydroxy-3-methoxyisoflavone.
    Relation: Molecules, v.22, n.1, UNSP 87
    Appears in Collections:[Dept. of Biotechnology (including master's program)] Periodical Articles

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