Production and Anti-Melanoma Activity of Methoxyisoflavones from the Biotransformation of Genistein by Two Recombinant Escherichia coli Strains

doi:10.3390/molecules22010087

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Title:	Production and Anti-Melanoma Activity of Methoxyisoflavones from the Biotransformation of Genistein by Two Recombinant Escherichia coli Strains
Authors:	Chiang, Chien-Min Chang, Yu-Jhe Wu, Jiumn-Yih Chang, Te-Sheng
Contributors:	Chia Nan Univ Pharm & Sci, Dept Biotechnol Natl Univ Tainan, Dept Biol Sci & Technol I Shou Univ, Inst Biotechnol & Chem Engn Natl Quemoy Univ, Ctr Gen Educ
Keywords:	genistein melanoma methoxyisoflavone methyltransferase tyrosinase
Date:	2017-01
Issue Date:	2018-11-30 15:54:32 (UTC+8)
Publisher:	Mdpi Ag
Abstract:	Biotransformation of the soy isoflavone genistein by sequential 3-hydroxylation using recombinant Escherichia coli expressing tyrosinase from Bacillus megaterium and then methylation using another recombinant E. coli expressing O-methyltransferase from Streptomyces peucetius was conducted. The results showed that two metabolites were produced from the biotransformation, identified as 5,7,4-trihydroxy-3-methoxyisoflavone and 5,7,3-trihydroxy-4-methoxyisoflavone, respectively, based on their mass and nuclear magnetic resonance spectral data. 5,7,4-Trihydroxy-3-methoxyisoflavone showed potent antiproliferative activity toward mouse B16 melanoma cells with an IC50 value of 68.8 M. In contrast, the compound did not show any cytotoxicity toward mouse normal fibroblast cells, even at 350 M concentration. The results of the present study offer insight on the production of both 5,7,4-trihydroxy-3-methoxyisoflavone and 5,7,3-trihydroxy-4-methoxyisoflavone by two recombinant E. coli strains and the potential anti-melanoma applications of 5,7,4-trihydroxy-3-methoxyisoflavone.
Relation:	Molecules, v.22, n.1, UNSP 87
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles

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