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| 標題: | Overexpression of a three-gene conidial pigment biosynthetic pathway in Aspergillus nidulans reveals the first NRPS known to acetylate tryptophan |
| 作者: | Sung, Calvin T.
Chang, Shu-Lin
Entwistle, Ruth
Ahn, Green
Lin, Tzu-Shyang
Petrova, Vessela
Yeh, Hsu-Hua
Praseuth, Mike B.
Chiang, Yi-Ming
Oakley, Berl R.
Wang, Clay C. C. |
| 貢獻者: | Univ Southern Calif, Sch Pharm, Dept Pharmacol & Pharmaceut Sci
Univ Kansas, Dept Mol Biosci
Chia Nan Univ Pharm & Sci, Dept Pharm
Univ Southern Calif, Dornsife Coll Letters Arts & Sci, Dept Chem
Chia Nan Univ Pharm & Sci, Drug Discovery & Dev Ctr
Chia Nan Univ Pharm & Sci, Dept Cosmet Sci |
| 關鍵字: | Aspergillus nidulans
Nonribosomal peptide synthetase (NRPS)
Pigment biosynthesis
Tryptophan N-acetyltransferase
N-acetyltryptophan oxidase |
| 日期: | 2017-04 |
| 上傳時間: | 2018-11-30 15:51:40 (UTC+8) |
| 出版者: | Academic Press Inc Elsevier Science |
| 摘要: | Fungal nonribosomal peptide synthetases (NRPSs) are megasynthetases that produce cyclic and acyclic peptides. In Aspergillus nidulans, the NRPS ivoA (AN10576) has been associated with the biosynthesis of grey-brown conidiophore pigments. Another gene, ivoB (AN0231), has been demonstrated to be an N-acetyl-6-hydroxytryptophan oxidase that putatively acts downstream of IvoA. A third gene, ivoC, has also been predicted to be involved in pigment biosynthesis based on publicly available genomic and transcriptomic information. In this paper, we report the replacement of the promoters of the ivoA, ivoB, and ivoC genes with the inducible promoter alcA in a single cotransformation. Co-overexpression of the three genes resulted in the production of a dark-brown pigment in hyphae. In addition, overexpression of each of the Ivo genes, ivoA-C, individually or in combination, allowed us to isolate intermediates and confirm the function of each gene. IvoA was found to be the first known NRPS to carry out the acetylation of the amino acid, tryptophan. (C) 2017 Elsevier Inc. All rights reserved. |
| 關聯: | Fungal Genetics and Biology, v.101, pp.1-6 |
| 顯示於類別: | [藥學系(所)] 期刊論文
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