Overexpression of a three-gene conidial pigment biosynthetic pathway in Aspergillus nidulans reveals the first NRPS known to acetylate tryptophan

doi:10.1016/j.fgb.2017.01.006

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Title:	Overexpression of a three-gene conidial pigment biosynthetic pathway in Aspergillus nidulans reveals the first NRPS known to acetylate tryptophan
Authors:	Sung, Calvin T. Chang, Shu-Lin Entwistle, Ruth Ahn, Green Lin, Tzu-Shyang Petrova, Vessela Yeh, Hsu-Hua Praseuth, Mike B. Chiang, Yi-Ming Oakley, Berl R. Wang, Clay C. C.
Contributors:	Univ Southern Calif, Sch Pharm, Dept Pharmacol & Pharmaceut Sci Univ Kansas, Dept Mol Biosci Chia Nan Univ Pharm & Sci, Dept Pharm Univ Southern Calif, Dornsife Coll Letters Arts & Sci, Dept Chem Chia Nan Univ Pharm & Sci, Drug Discovery & Dev Ctr Chia Nan Univ Pharm & Sci, Dept Cosmet Sci
Keywords:	Aspergillus nidulans Nonribosomal peptide synthetase (NRPS) Pigment biosynthesis Tryptophan N-acetyltransferase N-acetyltryptophan oxidase
Date:	2017-04
Issue Date:	2018-11-30 15:51:40 (UTC+8)
Publisher:	Academic Press Inc Elsevier Science
Abstract:	Fungal nonribosomal peptide synthetases (NRPSs) are megasynthetases that produce cyclic and acyclic peptides. In Aspergillus nidulans, the NRPS ivoA (AN10576) has been associated with the biosynthesis of grey-brown conidiophore pigments. Another gene, ivoB (AN0231), has been demonstrated to be an N-acetyl-6-hydroxytryptophan oxidase that putatively acts downstream of IvoA. A third gene, ivoC, has also been predicted to be involved in pigment biosynthesis based on publicly available genomic and transcriptomic information. In this paper, we report the replacement of the promoters of the ivoA, ivoB, and ivoC genes with the inducible promoter alcA in a single cotransformation. Co-overexpression of the three genes resulted in the production of a dark-brown pigment in hyphae. In addition, overexpression of each of the Ivo genes, ivoA-C, individually or in combination, allowed us to isolate intermediates and confirm the function of each gene. IvoA was found to be the first known NRPS to carry out the acetylation of the amino acid, tryptophan. (C) 2017 Elsevier Inc. All rights reserved.
Relation:	Fungal Genetics and Biology, v.101, pp.1-6
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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