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| 標題: | Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-beta/c-Fos-MMP-7 Pathways |
| 作者: | Chiu, Kun-Yuan
Chen, Tzu-Hsiu
Wen, Chi-Luan
Lai, Jin-Mei
Cheng, Chi-Chih
Liu, Hsiang-Chun
Hsu, Shih-Lan
Tzeng, Yew-Min |
| 貢獻者: | Chaoyang Univ Technol, Inst Biochem Sci & Technol
Taichung Vet Gen Hosp, Dept Surg, Div Urol
Natl Chi Nan Univ, Dept Appl Chem
Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr
Council Agr, Propagat Technol Sect, Taiwan Seed Improvement & Propagat Stn
Fu Jen Catholic Univ, Dept Life Sci
Taichung Vet Gen Hosp, Dept Med Res
Natl Taitung Univ, Dept Life Sci |
| 關鍵字: | Focal Adhesion Kinase
Nf-Kappa-B
Matrix Metalloproteinase-7
Tissue Inhibitor
Tyrosine Phosphorylation
Clinical-Significance
Ap-1 Activation
Fak Expression
Carcinoma
Metastasis |
| 日期: | 2017 |
| 上傳時間: | 2018-11-30 15:51:22 (UTC+8) |
| 出版者: | Hindawi Ltd |
| 摘要: | Antcin-H, a natural triterpene, is purified from a famous anticancer medicinal mushroom, Antrodia cinnamomea, in Taiwan. This study showed that antcin-H inhibited the growth of human renal carcinoma 786-0 cells; the IC50 value (for 48 h) was 170 mu M. Besides, the migration and invasion of 786-0 cells were suppressed by antcin-H under noncytotoxic concentrations (<100 mu M); these events were accompanied by inhibition of FAK and Src kinase activities, decrease of paxillin phosphorylation, impairment of lamellipodium formation, and upregulation of TIMPs and downregulation of MMPs, especially MMP-7 expression. Luciferase reporter assay showed that antcin-H repressed the MMP-7 promoter activity, in parallel to inhibiting c-Fos/AP-1 and C/EBP-beta transactivation abilities. Moreover, antcin-H suppressed the activity of ERK1/2 and decreased the binding ability of C/EBP-beta and c-Fos on the upstream/enhancer region of MMP-7 promoter. Overall, this study demonstrated that the anti-invasive effect of antcin-H in human renal carcinoma 786-0 cells might be at least in part by abrogating focal adhesion complex and lamellipodium formation through inhibiting the Src/FAK-paxillin signaling pathways and decreasing MMP-7 expression through suppressing the ERK1/2-AP-1/c-Fos and C/EBP-beta signaling axis. Our findings provide the evidence that antcin-H may be an active component existing in A. cinnamomea with anticancer effect. |
| 關聯: | Evidence-Based Complementary and Alternative Medicine, v.2017, 文獻號碼:5052870 |
| 顯示於類別: | [保健營養系(所) ] 期刊論文
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