Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-beta/c-Fos-MMP-7 Pathways

doi:10.1155/2017/5052870

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/31645

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Title:	Antcin-H Isolated from Antrodia cinnamomea Inhibits Renal Cancer Cell Invasion Partly through Inactivation of FAK-ERK-C/EBP-beta/c-Fos-MMP-7 Pathways
Authors:	Chiu, Kun-Yuan Chen, Tzu-Hsiu Wen, Chi-Luan Lai, Jin-Mei Cheng, Chi-Chih Liu, Hsiang-Chun Hsu, Shih-Lan Tzeng, Yew-Min
Contributors:	Chaoyang Univ Technol, Inst Biochem Sci & Technol Taichung Vet Gen Hosp, Dept Surg, Div Urol Natl Chi Nan Univ, Dept Appl Chem Chia Nan Univ Pharm & Sci, Dept Hlth & Nutr Council Agr, Propagat Technol Sect, Taiwan Seed Improvement & Propagat Stn Fu Jen Catholic Univ, Dept Life Sci Taichung Vet Gen Hosp, Dept Med Res Natl Taitung Univ, Dept Life Sci
Keywords:	Focal Adhesion Kinase Nf-Kappa-B Matrix Metalloproteinase-7 Tissue Inhibitor Tyrosine Phosphorylation Clinical-Significance Ap-1 Activation Fak Expression Carcinoma Metastasis
Date:	2017
Issue Date:	2018-11-30 15:51:22 (UTC+8)
Publisher:	Hindawi Ltd
Abstract:	Antcin-H, a natural triterpene, is purified from a famous anticancer medicinal mushroom, Antrodia cinnamomea, in Taiwan. This study showed that antcin-H inhibited the growth of human renal carcinoma 786-0 cells; the IC50 value (for 48 h) was 170 mu M. Besides, the migration and invasion of 786-0 cells were suppressed by antcin-H under noncytotoxic concentrations (<100 mu M); these events were accompanied by inhibition of FAK and Src kinase activities, decrease of paxillin phosphorylation, impairment of lamellipodium formation, and upregulation of TIMPs and downregulation of MMPs, especially MMP-7 expression. Luciferase reporter assay showed that antcin-H repressed the MMP-7 promoter activity, in parallel to inhibiting c-Fos/AP-1 and C/EBP-beta transactivation abilities. Moreover, antcin-H suppressed the activity of ERK1/2 and decreased the binding ability of C/EBP-beta and c-Fos on the upstream/enhancer region of MMP-7 promoter. Overall, this study demonstrated that the anti-invasive effect of antcin-H in human renal carcinoma 786-0 cells might be at least in part by abrogating focal adhesion complex and lamellipodium formation through inhibiting the Src/FAK-paxillin signaling pathways and decreasing MMP-7 expression through suppressing the ERK1/2-AP-1/c-Fos and C/EBP-beta signaling axis. Our findings provide the evidence that antcin-H may be an active component existing in A. cinnamomea with anticancer effect.
Relation:	Evidence-Based Complementary and Alternative Medicine, v.2017, 文獻號碼:5052870
Appears in Collections:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

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