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| 標題: | Enhancement of microbial 2,4,6-trinitrotoluene transformation with increased toxicity by exogenous nutrient amendment |
| 作者: | Liang, Shih-Hsiung
Hsu, Duen-Wei
Lin, Chia-Ying
Kao, Chih-Ming
Huang, Da-Ji
Chien, Chih-Ching
Chen, Ssu-Ching
Tsai, Isheng Jason
Chen, Chien-Cheng |
| 貢獻者: | Natl Kaohsiung Normal Univ, Dept Biotechnol
Natl Sun Yat Sen Univ, Inst Environm Engn
Chia Nan Univ Pharm & Sci, Dept Environm Resources Management
Yuan Ze Univ, Grad Sch Biotechnol & Bioengn
Natl Cent Univ, Dept Life Sci
Acad Sinica, Biodivers Res Ctr |
| 關鍵字: | 2;4;6-trinitrotoluene (TNT)
Transformation
Bioremediation
Cometabolism
Nitroreductase
Toxicity |
| 日期: | 2017-04 |
| 上傳時間: | 2018-11-30 15:50:55 (UTC+8) |
| 出版者: | Academic Press Inc Elsevier Science |
| 摘要: | In this study, the bacterial strain Citrobacter youngae strain E4 was isolated from 2,4,6-trinitrotoluene (TNT) contaminated soil and used to assess the capacity of TNT transformation with/without exogenous nutrient amendments. C. youngae E4 poorly degraded TNT without an exogenous amino nitrogen source, whereas the addition of an amino nitrogen source considerably increased the efficacy of TNT transformation in a dose dependent manner. The enhanced TNT transformation of C. youngae E4 was mediated by increased cell growth and up-regulation of TNT nitroreductases, including NemA, NfsA and NfsB. This result indicates that the increase in TNT transformation by C. youngae E4 via nitrogen nutrient stimulation is a cometabolism process. Consistently, TNT transformation was effectively enhanced when C. youngae E4 was subjected to a TNT contaminated soil slurry in the presence of an exogenous amino nitrogen amendment. Thus, effective enhancement of TNT transformation via the coordinated inoculation of the nutrient-responsive C. youngae E4 and an exogenous nitrogen amendment might be applicable for the remediation of TNT-contaminated soil. Although the TNT transformation was significantly enhanced by C. youngae E4 in concert with biostimulation, the 96-h LC50 value of the TNT transformation product mixture on the aquatic invertebrate Tigriopus japonicas was higher than the LC50 value of TNT alone. Our results suggest that exogenous nutrient amendment can enhance microbial TNT transformation; however, additional detoxification processes may be needed due to the increased toxicity after reduced TNT transformation. |
| 關聯: | Ecotoxicology and Environmental Safety, v.138, pp.39-46 |
| 顯示於類別: | [環境資源管理系(所)] 期刊論文
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