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| 標題: | Solasodine inhibits invasion of human lung cancer cell through downregulation of miR-21 and MMPs expression |
| 作者: | Shen, Kun-Hung
Hung, Jui-Hsiang
Chang, Chia-Wei
Weng, Yu-Ting
Wu, Ming-Jiuan
Chen, Pin-Shern |
| 貢獻者: | Chi Mei Med Ctr, Div Urol, Dept Surg
Southern Taiwan Univ Sci & Technol, Ctr Gen Educ
Taipei Med Univ, Dept Urol
Chia Nan Univ Pharm & Sci, Dept Biotechnol |
| 關鍵字: | Solasodine
Lung cancer
Cell invasion
Matrix metalloproteinase
MicroRNA-21 |
| 日期: | 2017-04-25 |
| 上傳時間: | 2018-11-30 15:50:20 (UTC+8) |
| 出版者: | Elsevier Ireland Ltd |
| 摘要: | Solasodine, a naturally occurring aglycone of glycoalkaloid in eggplant (Solanum melongena), was found to inhibit proliferation in various tumor cells. However, the effect of solasodine on cancer cell metastasis remains unclear. This study investigates the suppression mechanism of solasodine on motility of human lung cancer cell A549 in vitro. Results show that solasodine reduces viability of A549 cells. Treatment with non-toxic doses of solasodine suppresses markedly cell invasion. Solasodine reduces the mRNA level of matrix metalloproteinase-2 (MMP-2), MMP-9 and extracellular inducer of matrix metalloproteinase (EMMPRIN), but increases the expression of reversion-inducing cysteine-rich protein with kazal motifs (RECK), as well as tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2. Immunoblotting assays indicate that solasodine is effective in suppressing PI3K and Akt phosphorylation. Moreover, solasodine downregulates oncogenic microRNA-21 (miR-21), which has been known to target RECK. Downregulation of miR-21 by miR-21 inhibitor increases RECK expression and decreases cell invasion, suggesting that downregulation of miR-21 by solasodine may contribute to elevate RECK expression and subsequently inhibiting cell invasion. Taken together, the results reveal that inhibition of A549 cell invasion by solasodine may be, at least in part, through blocking MMP expression. Solasodine also reduces PI3K/Akt signaling pathways and downregulates expression of miR-21. These findings demonstrate an attractive therapeutic potential for solasodine in lung cancer anti-metastatic therapy. (C) 2017 Elsevier B.V. All rights reserved. |
| 關聯: | Chemico-Biological Interactions, v.268, pp.129-135 |
| 顯示於類別: | [生物科技系(所)] 期刊論文
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