Solasodine inhibits invasion of human lung cancer cell through downregulation of miR-21 and MMPs expression

doi:10.1016/j.cbi.2017.03.005

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標題:	Solasodine inhibits invasion of human lung cancer cell through downregulation of miR-21 and MMPs expression
作者:	Shen, Kun-Hung Hung, Jui-Hsiang Chang, Chia-Wei Weng, Yu-Ting Wu, Ming-Jiuan Chen, Pin-Shern
貢獻者:	Chi Mei Med Ctr, Div Urol, Dept Surg Southern Taiwan Univ Sci & Technol, Ctr Gen Educ Taipei Med Univ, Dept Urol Chia Nan Univ Pharm & Sci, Dept Biotechnol
關鍵字:	Solasodine Lung cancer Cell invasion Matrix metalloproteinase MicroRNA-21
日期:	2017-04-25
上傳時間:	2018-11-30 15:50:20 (UTC+8)
出版者:	Elsevier Ireland Ltd
摘要:	Solasodine, a naturally occurring aglycone of glycoalkaloid in eggplant (Solanum melongena), was found to inhibit proliferation in various tumor cells. However, the effect of solasodine on cancer cell metastasis remains unclear. This study investigates the suppression mechanism of solasodine on motility of human lung cancer cell A549 in vitro. Results show that solasodine reduces viability of A549 cells. Treatment with non-toxic doses of solasodine suppresses markedly cell invasion. Solasodine reduces the mRNA level of matrix metalloproteinase-2 (MMP-2), MMP-9 and extracellular inducer of matrix metalloproteinase (EMMPRIN), but increases the expression of reversion-inducing cysteine-rich protein with kazal motifs (RECK), as well as tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2. Immunoblotting assays indicate that solasodine is effective in suppressing PI3K and Akt phosphorylation. Moreover, solasodine downregulates oncogenic microRNA-21 (miR-21), which has been known to target RECK. Downregulation of miR-21 by miR-21 inhibitor increases RECK expression and decreases cell invasion, suggesting that downregulation of miR-21 by solasodine may contribute to elevate RECK expression and subsequently inhibiting cell invasion. Taken together, the results reveal that inhibition of A549 cell invasion by solasodine may be, at least in part, through blocking MMP expression. Solasodine also reduces PI3K/Akt signaling pathways and downregulates expression of miR-21. These findings demonstrate an attractive therapeutic potential for solasodine in lung cancer anti-metastatic therapy. (C) 2017 Elsevier B.V. All rights reserved.
關聯:	Chemico-Biological Interactions, v.268, pp.129-135
显示于类别:	[生物科技系(所)] 期刊論文

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