Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/31023
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    標題: Architecture effects of glucose oxidase/Au nanoparticle composite Langmuir-Blodgett films on glucose sensing performance
    作者: Wang, Ke-Hsuan
    Wu, Jau-Yann
    Chen, Liang-Huei
    Lee, Yuh-Lang
    貢獻者: Natl Cheng Kung Univ, Dept Chem Engn
    I Shou Univ, Dept Chem Engn
    Chia Nan Univ Pharm & Sci, Dept Med Chem
    關鍵字: Langmuir-Blodgett films
    Glucose oxidase
    Gold nanoparticles
    Composite film
    Architecture effect
    immobilization
    日期: 2016-03
    上傳時間: 2018-01-18 11:40:03 (UTC+8)
    出版者: Elsevier Science Bv
    摘要: The Langmuir-Blodgett (LB) deposition technique is employed to prepare nano-composite films consisting of glucose oxidase (GOx) and gold nanoparticles (AuNPs) for glucose sensing applications. The GOx and AuNPs are co-adsorbed from an aqueous solution onto an air/liquid interface in the presence of an octadecylamine ( ODA) template monolayer, forming a mixed (GOx-AuNP) monolayer. Alternatively, a composite film with a cascade architecture (AuNP/G0x) is also prepared by sequentially depositing monolayers of AuNPs and GOx. The architecture effects of the composite LB films on the glucose sensing are studied. The results show that the presence of AuNPs in the co-adsorption system does not affect the adsorption amount and preferred conformation (alpha-helix) of GOx. Furthermore, the incorporation of AuNPs in both composite films can significantly improve the sensing performance. However, the enhancement effects of the AuNPs in the two architectures are distinct. The major effect of the AuNPs is on the facilitation of charge-transfer in the (GOx-AuNP) film, but on the increase of catalytic activity in the (AuNP/G0x) one. Therefore, the sensing performance can be greatly improved by utilizing a film combining both architectures (AuNP/G0x-AuNP). (C) 2016 Elsevier B.V. All rights reserved.
    關聯: Applied Surface Science, v.366, pp.202-209
    显示于类别:[食藥產業暨檢測科技系(含五專)] 期刊論文

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